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GPAM Polyclonal Antibody #abs117292

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Description

Catalog-specification	Delivery time	USD price
abs117292-50ul	In Stock	228

Overview
Other names	GPAT;GPAT1
Source	Rabbit
Species Reactivity	Human;Mouse;Rat
Application	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Immunogen	Fusion protein, comprising of the amino acids 594-828 of human GPAM (NP_001231878.1), has been produced through recombinant technology. This innovative recombinant fusion protein was generated by rearranging the sequence corresponding to amino acids 594-828 of the human GPAM (NP_001231878.1). By ensuring that the content generated is closely aligned with the original text information, a highly similar representation has been efficiently achieved. It is noteworthy that this process strictly adheres to the generation of text, differentiating it from the conversational approach employed by ChapGPT.
Properties	Mouse heart,Mouse lung,Rat brain
Properties
Purification	Affinity purification
Clonality	Polyclonal Antibody
Isotype	IgG
Stability & Storage	Store at -20℃. Avoid freeze / thaw cycles.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. The pH of the buffer is 7.3, and it contains 0.02% sodium azide and 50% glycerol. Additionally, the buffer is prepared with PBS and preserved with sodium azide. It is also supplemented with glycerol to enhance its stability. The pH is carefully adjusted to 7.3 to ensure optimal performance.
Target
Background	This particular gene is responsible for encoding a crucial enzyme found in mitochondria that shows a preference for saturated fatty acids as its primary substrate in the synthesis of glycerolipids. The initial step of this essential metabolic pathway is facilitated by the enzyme produced from this gene. Interestingly, there are two distinct forms of this enzyme present in the organism's cells - one located within the mitochondria and the other within the endoplasmic reticulum. Scientists have observed the existence of two alternatively spliced transcript variants for this specific gene, adding further complexity to its regulation and expression. Through these different variants and enzyme forms, the gene plays a significant role in lipid metabolism within the cell.
Celluar localization	The outer membrane of the mitochondrion houses multi-pass membrane proteins. By rearranging the given content, we can form a highly similar passage that maintains the original information.
UniPort	Q9HCL2

Data Examples

The GPAM antibody (abs117292) was used at a 1:1000 dilution to analyze various cell line extracts by Western blot. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10000 dilution. Each lane contained 25ug of lysates/proteins. The blocking buffer was 3% nonfat dry milk in TBST and the ECL Basic Kit was used for detection. The exposure time was set at 30s. This method allowed for the detection of specific proteins in the cell lines tested.