Rabbit Anti-HDAC6 Polyclonal Antibody #abs130445

abs130445-50ug

1-2 Weeks

201

abs130445-100ug

1-2 Weeks

301

Description

Histones are crucial for regulating transcription, cell cycle progression, and developmental processes. Chromosome structure and transcription factor access to DNA are altered by histone acetylation/deacetylation. This gene encodes a protein belonging to class II of the histone deacetylase/acuc/apha family. It consists of two duplicated catalytic domains that seem to function independently. With its histone deacetylase activity, this protein can suppress transcription. Let's rearrange the content and provide a highly similar version:
Transcriptional regulation, cell cycle progression, and developmental events heavily rely on the role of histones. By modifying chromosome structure and influencing DNA access for transcription factors, histone acetylation or deacetylation plays a vital part. In the histone deacetylase/acuc/apha family, this gene encodes a protein belonging to class II. It possesses two catalytic domains that are internally duplicated but function autonomously. Through its histone deacetylase activity, this protein effectively represses transcription.

Other names

The protein encoded by the gene CPBHM, also known as FLJ16239 or HDAC6, is a histone deacetylase. Specifically, it is the histone deacetylase 6, also called HD6, which is involved in the regulation of gene expression. Additionally, this protein is also known as KIAA0901 and JM21. It has two alternative names, namely OTTHUMP00000032398 and OTTHUMP00000197663, and is a regulatory subunit of protein phosphatase 1 (PPP1R90). This protein has been shown to have a crucial role in a variety of cellular functions in humans.

Source

Rabbit

Specificity

HDAC6 Antibody detects endogenous levels of HDAC6

Species Reactivity

Human;Mouse

Application

The recommended dilution range for WB is 1:500-1:2000, while for IHC, it is 1:50-1:200. For ELISA using peptide, the suggested dilution range is 1:20000-1:40000. These dilution ranges ensure optimal results for each application, allowing for accurate and reliable detection of the target protein. It is important to follow these guidelines to obtain the best possible outcome.

Immunogen

A synthesized peptide derived from human HDAC6

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

It is recommended to store the product at a temperature of -20 °C for a period of one year. However, it is important to avoid any kind of repeated freeze and thaw cycles to ensure the optimal quality of the product.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline at pH 7.4 with 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended to store it at -20 °C and it is stable for up to 12 months from the date of receipt. Please take note of the storage conditions to ensure the product's integrity.

Target

Background

The deacetylation of lysine residues on the N-terminal region of core histones (H2A, H2B, H3, and H4) is under the responsibility of this entity. This process of histone deacetylation instigates an epigenetic repression signal and plays a significant role in regulating transcription, advancing cell cycle progression, and orchestrating developmental events. Through the formation of extensive multiprotein complexes, histone deacetylases exert their actions in this regard (as suggested by similarities with other related entities). By deacetylating tubulin, it assumes a pivotal role in facilitating microtubule-mediated cellular motility. Furthermore, it participates in the regulation of ESR1 expression in breast cancer via its interaction with MTA1, thereby contributing to epigenetic alterations in gene expression.

Posttranslational modification

Phosphorylated by AURKA.Ubiquitinated. Its polyubiquitination however does not lead to its degradation.Sumoylated in vitro.

Celluar localization

Cytoskeleton;Cytosol;Endosome;Nucleus;Plasma Membrane;

UniPort

Q9UBN7