Rabbit Anti-Gastrin Polyclonal Antibody #abs130444

abs130444-50ug

1-2 Weeks

201

abs130444-100ug

1-2 Weeks

301

Description

Gastrin, a hormone belonging to the gastrin/cholecystokinin family, plays a vital role in stimulating various functions within the digestive system. Its primary function is to stimulate the stomach mucosa to produce and release hydrochloric acid, necessary for the process of digestion. Additionally, it stimulates the pancreas to secrete digestive enzymes, aiding in the breakdown of food. Gastrin also influences the contraction of smooth muscles and promotes increased blood circulation and water secretion in both the stomach and intestine. Its multifaceted actions contribute to the efficient functioning of the digestive system.

Other names

Gastrin is a hormone that regulates the production of acid in the stomach. It is made up of several components, including Gastrin component I, II, and III, and precursors Gastrin-14, -17, -34, -52, -6, and -71. While they have different lengths, they all play a significant role in the digestive process. Gastrin-14, -17, and -34 stimulate the release of gastric acid and pepsin, while Gastrin-52 and -71 are involved in the growth and division of cells in the digestive tract. GAS, Gast, and GAST_HUMAN also refer to Gastrin in various contexts. Overall, Gastrin is a multifunctional hormone essential for proper digestion.

Source

Rabbit

Specificity

The detection of endogenous levels of Gastrin can be achieved using Gastrin Antibody.

Species Reactivity

Human;Mouse;Rat

Application

For western blotting, the recommended dilution range is 1:500 to 1:2000, while for immunohistochemistry it is 1:50 to 1:200. In terms of IF/ICC, a dilution range of 1:100 to 1:500 is suggested. ELISA testing using peptide should be performed with a dilution range of 1:20000 to 1:40000 for optimal results. These dilution guidelines are based on the original text information and should be followed for the most effective experimental outcomes.

Immunogen

A synthesized peptide derived from human Gastrin.

Properties

Concentration

1mg/ml

Purification

To produce highly purified antiserum, peptide affinity chromatography was employed with the use of SulfoLink™ Coupling Resin.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the integrity of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is crucial to avoid subjecting it to repeated freeze and thaw cycles.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline with pH 7.4, 150mM NaCl, and 0.02% sodium azide. It also has a 50% glycerol content and should be stored at a temperature of -20 °C for optimal preservation. The solution is stable for a period of 12 months from the date of receiving the product.

Target

Background

When gastrin is released, it triggers the stomach lining to generate and release hydrochloric acid, as well as prompting the pancreas to secrete its digestive enzymes. Additionally, it stimulates the contraction of smooth muscles, enhances blood circulation, and promotes the secretion of water in both the stomach and intestine.

Posttranslational modification

Antral G-cells in the stomach are believed to possess two distinct processing pathways. The first and more prominent pathway involves the cleavage of progastrin at three different sites. This cleavage process generates two major types of biologically active gastrins: gastrin-34 and gastrin-17. Meanwhile, an alternative processing pathway may exist where progastrin is only cleaved at the most C-terminal dibasic site, leading to the production of gastrin-71.
A noteworthy factor that influences the processing of gastrins is sulfation. Sulfation serves to enhance proteolytic processing while simultaneously preventing peptide degradation. The level of sulfation varies among the different gastrin molecules that undergo proteolytic cleavage. For instance, gastrin-6 exhibits a remarkably high sulfation level of nearly 73%, whereas the larger gastrins have sulfation levels below 50%. Interestingly, the degree of sulfation also differs depending on the specific tissue involved.
To summarize, antral G-cells possess multiple processing pathways for progastrin, resulting in the generation of distinct bioactive gastrins. Sulfation plays a crucial role in modulating both proteolytic processing and peptide degradation, with variations in sulfation levels observed among different gastrin molecules and tissues.

Celluar localization

Extracellular region or secreted;

UniPort

P01350