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Rabbit Anti-NCF1 Polyclonal Antibody#abs135802

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Description

Catalog-specification	Delivery time	USD price
abs135802-100ug	1-2 Weeks	301.0
abs135802-50ug	1-2 Weeks	201.0

Overview
catalog	abs135802
Description	NADPH oxidase, an essential enzyme in the body's defense against pathogens, functions as a phagocytic agent by reducing oxygen to superoxide. Comprising of six subunits, including p91 phox and p22 phox heterodimers (known as cytochrome b558) on the membrane, and the cytosolic complex of p40phox, p47phox, and p67phox, as well as the small GTPase Rac2, NADPH oxidase plays a crucial role in combating invading pathogens. The activation process involves the phosphorylation of the cytosolic complex, leading to a conformational change that triggers the translocation of the complex to the membrane, forming an active enzyme with cytochrome b558. Interestingly, defects in the p47phox subunit, primarily caused by recombination events with a pseudogene, can result in chronic granulomatous disease. Furthermore, heightened oxidative stress due to increased activity of NADPH oxidase in the myocardium has been identified as a potential contributor to heart failure. Thus, understanding the intricate workings of NADPH oxidase is crucial in both immunological responses and cardiovascular health.
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Source	Rabbit
Specificity	The NCF1 antibody is capable of detecting the complete amount of endogenous NCF1 found within an organism. This information indicates that the antibody can be used to measure the levels of total NCF1 present in a sample.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine
Antigen	NCF1
Application	The recommended dilutions for using the antibody in different applications are as follows: A dilution of 1:500 to 1:2000 is recommended for western blotting, 1:50 to 1:200 for use in immunohistochemistry, while a dilution of 1:100 to 1:500 is recommended for immunofluorescence/immunocytochemistry. For ELISA with peptide, it is recommended to use a dilution of 1:20000 to 1:40000. These dilutions have been optimized for best results and should be used as a guideline.
Immunogen	A synthesized peptide derived from human NCF1.
MW	45kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Tissue specificity	Detected in peripheral blood monocytes and neutrophils (at protein level).
Posttranslational modification	Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.
Celluar localization	Cytoskeleton;Cytosol;Endoplasmic reticulum;Golgi apparatus;Lysosome;Plasma Membrane;
UniPort	P14598