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Rabbit Anti-IRF5 Polyclonal Antibody#abs135803

Please note that the price mentioned serves only as a reference point. For more details on the exact price of our products, kindly get in touch with our representative Vecent. Western blot analysis of extracts of various celllines, using IRF5 antibody. This product is for research use only, not...

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Description

Catalog-specification	Delivery time	USD price
abs135803-100ug	1-2 Weeks	301.0
abs135803-50ug	1-2 Weeks	201.0

Please note that the price mentioned serves only as a reference point. For more details on the exact price of our products, kindly get in touch with our representative Vecent.

Overview
catalog	abs135803
Description	The family of transcription factors known as interferon regulatory factors (IRFs) play a pivotal role in regulating interferon expression and IFN-inducible genes when the host encounters viral infections. IRFs are also involved in the defense against pathogens, lymphocyte development, autoimmunity, cell growth, and susceptibility to transformation. IRFs comprise nine members, namely IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP, and they all possess a similar DNA-binding motif in their amino-terminal domain. These proteins associate in transcription regulation through interactions with proteins that share DNA-binding motifs. IRF-5 is highly expressed in lymphoid tissues and peripheral blood lymphocytes and is vital in the induction of type I interferon genes upon viral infection. Adversely, the IRF-5 signaling pathway activates by different TLRs, including TLR7 and MyD88. Genetic IRF-5 variants have been linked with immune disorders consequent to IFN pathway hyperactivity, including systemic lupus erythematosus.
Other names	IRF-5, also known as Interferon regulatory factor 5 or IRF5, is a gene that encodes a protein involved in the regulation of interferon production and immune response. The bone marrow variant of IRF-5 has been identified as a potential factor in the development of certain autoimmune disorders, including systemic lupus erythematosus (SLE). IRF-5 plays a crucial role in the activation of various immune cells and the production of cytokines, which are important mediators of inflammation. Understanding the function and regulation of IRF-5 could provide valuable insights into the pathogenesis of autoimmune diseases and potentially lead to the development of new therapeutic strategies. The study of IRF-5, both its normal and variant forms, is an active area of research in the field of immunology and genetics.
Source	Rabbit
Specificity	The IRF5 Antibody is designed to recognize and bind to total IRF5 that is present within the cells naturally. This antibody is useful in detecting endogenous levels of IRF5 and can be used in a variety of experimental settings. By recognizing the total amount of IRF5, this antibody can help researchers study the role of this protein in various cellular processes and disease states.
Species Reactivity	Human;Mouse;Rat
Antigen	IRF5
Application	The suggested dilution ranges for WB, IHC, and ELISA(peptide) assays are as follows: WB should be diluted in the range of 1:500 to 1:2000, IHC should be diluted in the range of 1:50 to 1:200, and ELISA(peptide) should be diluted in the range of 1:20000 to 1:40000. These dilution ranges are commonly used to achieve optimal results in these assays.
Immunogen	A synthesized peptide derived from human IRF5.
MW	56kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.
Posttranslational modification	Phosphorylation of serine and threonine residues in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/p300 and initiation of transcription.'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.
Celluar localization	Cytosol;Nucleus;
UniPort	Q13568