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Rabbit Anti-PGR Polyclonal Antibody#abs136349

Please be advised that the provided price is purely for your reference. For detailed pricing, we kindly request you to get in touch with our seller, Vecent. Western blot analysis of extracts of A431cell lines, using PGR antibody. This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs136349-50ug	1-2 Weeks	201.0
abs136349-100ug	1-2 Weeks	301.0

Please be advised that the provided price is purely for your reference. For detailed pricing, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs136349
Description	The progesterone receptor (PR) in humans is expressed as two different forms, PR A and PR B. PR A lacks the first 164 amino acids of PR B. Both forms are activated by ligands, but their ability to activate the transcription of target genes differs. Phosphorylation plays a crucial role in the regulation of PR activity, with at least seven serine residues in the amino-terminal domain being phosphorylated. Unique phosphorylation sites in PR B include Ser81, Ser102, and Ser162, while other sites, such as Ser190, Ser294, Ser345, and Ser400, are shared by both isoforms. CDK2 catalyzes the phosphorylation of Ser190 (which is equivalent to Ser26 of PR A). A mutation in Ser190 reduces the activity of PR, indicating its critical role in the biological function of PR.
Other names	The progesterone receptor, or PR, is also known as nuclear receptor subfamily 3 group C member 3, or NR3C3. There are two forms of the PR: progestin receptor form A, or PRA, and progestin receptor form B, or PRB. PR is responsible for binding to the hormone progesterone and triggering a cascade of physiological responses, such as cell growth and differentiation in the female reproductive system. Understanding the structure and function of PR is crucial for developing treatments for hormonally-related diseases such as breast cancer and endometriosis.
Source	Rabbit
Specificity	Endogenous levels of total PGR can be detected by using the PGR Antibody. The antibody effectively recognizes and binds to the protein, allowing for its detection in various biological samples. Through this detection method, researchers can gain insights into the expression levels and localization of PGR in their experimental systems.
Species Reactivity	Human;Mouse;Rat
Antigen	PGR
Application	For Western Blotting, a dilution range of 1:500 to 1:2000 is recommended. For Immunohistochemistry, a dilution range of 1:50 to 1:200 is recommended. For Immunofluorescence/Immunocytochemistry, a dilution range of 1:100 to 1:500 is recommended. And for ELISA using a peptide substrate, a dilution range of 1:20000 to 1:40000 is recommended. These dilutions will help ensure the best results for your experiments.
Immunogen	A synthetic peptideof human PGR.
MW	99kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, a peptide affinity chromatography method was employed to purify the antiserum. The resulting product was of high quality and exhibited excellent binding specificity.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor.
Tissue specificity	In reproductive tissues the expression of isoform A and isoform B varies as a consequence of developmental and hormonal status. Isoform A and isoform B are expressed in comparable levels in uterine glandular epithelium during the proliferative phase of the menstrual cycle. Expression of isoform B but not of isoform A persists in the glands during mid-secretory phase. In the stroma, isoform A is the predominant form throughout the cycle. Heterogeneous isoform expression between the glands of the endometrium basalis and functionalis is implying region-specific responses to hormonal stimuli.
Posttranslational modification	Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G2/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
Celluar localization	Cytosol;Mitochondrion;Nucleus;
UniPort	P06401