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Rabbit Anti-SIN3A Polyclonal Antibody#abs135993

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly ask you to get in touch with our seller, Vecent. Western blot analysis of extracts of various celllines, using SIN3A antibody. This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs135993-100ug	1-2 Weeks	301.0
abs135993-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly ask you to get in touch with our seller, Vecent.

Overview
catalog	abs135993
Description	Initially discovered as a transcriptional repressor in yeast, SIN3 (SIN3A and SIN3B) has since been identified in mammalian cells. These nuclear proteins act as scaffolding subunits for the SIN3 transcriptional repressor complex. The complex comprises SIN3A or SIN3B, along with HDAC1, HDAC2, SDS3, RBBP4/RBAP48, RBBP7/RBAP46, SAP30, and SAP18. Four paired amphipathic alpha-helix (PAH) motifs enable SIN3 proteins to recruit the complex to target genes by interacting with various DNA-binding transcriptional repressor proteins such as p53, E2F4, AML1, and more. The HDAC interaction domain (HID) facilitates the binding of SIN3 to HDAC1 and HDAC2 via SDS3, while the highly conserved region (HCR) at the carboxy terminus aids in repressor protein binding. Besides histone deacetylation at target gene promoters, SIN3 also plays a role in deacetylating histones in the coding regions, suppressing unwanted transcription by RNA polymerase II. Additionally, the SIN3 complex associates with various activities like histone methyltransferase, histone demethylase, chromatin remodeling, methylcytosine dioxygenase, and O-GlcNAc transferase, contributing to the regulation of target genes. Crucially, the SIN3 complex is involved in embryonic development, cell growth, apoptosis, DNA replication, DNA repair, and DNA methylation processes.
Other names	Histone deacetylase complex subunit Sin 3a, also known as Sin3a, is a transcriptional corepressor involved in gene regulation. It is encoded by several gene names, including AW553200, DKFZP434K2235, FLJ90319, KIAA0700, Kiaa4126, mKIAA4126, Paired amphipathic helix protein Sin3a, and SIN3 homolog A transcription regulator (yeast). Sin3a functions as a part of the histone deacetylase complex, which plays a role in modifying chromatin structure and regulating gene expression. Sin3a is responsible for recruiting histone deacetylases to target genes, leading to the removal of acetyl groups from histone proteins. This results in the repression of gene transcription. Sin3a contains paired amphipathic helix domains, which aid in protein-protein interactions and contribute to its transcriptional regulatory function. The SIN3A protein, expressed in humans, serves as a transcriptional co-repressor, interacting with various transcription factors and repressing gene expression. It acts as a bridge between DNA-binding transcription factors and histone-modifying enzymes, facilitating the recruitment of repressive chromatin remodeling complexes to specific gene promoters. Understanding the function and regulation of Sin3a is crucial for unraveling the complex mechanisms underlying gene expression and chromatin remodeling. Further research on Sin3a could provide insights into its potential roles in development, disease, and therapeutic interventions targeting epigenetic regulation.
Source	Rabbit
Specificity	SIN3A Antibody detects endogenous levels of total SIN3A.
Species Reactivity	Human;Mouse;Rat
Antigen	SIN3A
Application	WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human SIN3A.
MW	145kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in he control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (By similarity).
Posttranslational modification	SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2.
Celluar localization	Nucleus;
UniPort	Q96ST3