Rabbit Anti-STEAP4 Polyclonal Antibody #abs130430

abs130430-50ug

1-2 Weeks

201

abs130430-100ug

1-2 Weeks

301

Description

A member of the STEAP family, the metalloreductase STEAP4 plays a crucial role in maintaining systemic metabolic homeostasis by reducing both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+), utilizing NAD(+) as an acceptor. It intertwines the inflammatory and metabolic responses, making it a target for research in the context of obesity and insulin resistance. Moreover, STEAP4's involvement in regulating inflammatory cytokines has been linked to inflammatory arthritis. Interestingly, it exhibits the ability to inhibit anchorage-independent cell proliferation, highlighting its potential significance in cancer therapeutics.

Other names

Let me tell you about STEAP4, also known as alpha-induced protein 9 or TNFAIP9. This metalloreductase is a member of the STEAP family and has six transmembrane domains. Another name it goes by is Six-transmembrane epithelial antigen of prostate 4 or STAMP2. STEAP4 is involved in the reduction of metal ions and is highly expressed in adipose tissue. Its expression is induced by tumor necrosis factor alpha (TNF-alpha), hence the name TNFAIP9. It has also been implicated in prostate cancer progression and metastasis. Overall, STEAP4 is a fascinating protein with multiple roles in cellular processes.

Source

Rabbit

Specificity

The STEAP4 Antibody is capable of detecting STEAP4 levels that are naturally present in the body. To create similar content, the original text should be rearranged and the information should be conveyed using different words and structure.

Species Reactivity

Human

Application

The recommended dilutions for IHC are 1:50-1:200, for IF/ICC are 1:100-1:500, and for ELISA (peptide) are 1:20000-1:40000. It is important to follow these dilution guidelines to ensure accurate and reliable results when using these techniques. Dilution ranges are provided to give flexibility depending on the specific experiment or application.

Immunogen

A synthesized peptide derived from human STEAP4.

Properties

Concentration

1mg/ml

Purification

To purify the antiserum, SulfoLink™ Coupling Resin was employed for peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal storage conditions, it is recommended to keep the store at -20 °C for a duration of one year. It is important to avoid subjecting the stored items to repeated cycles of freezing and thawing.

Storage buffer

This Rabbit IgG comes in a phosphate buffered saline solution with pH 7.4, 150mM of NaCl, 0.02% of sodium azide, and 50% of glycerol. It should be stored at -20°C and is stable for up to 12 months from the date of receipt.

Target

Background

This metalloreductase exhibits the remarkable ability to reduce Fe3+ to Fe2+ as well as Cu2+ to Cu1+ (as per similarity). It utilizes NADP+ as an acceptor (as per similarity). Furthermore, this versatile enzyme plays a crucial role in maintaining systemic metabolic homeostasis by integrating inflammatory and metabolic responses (as per similarity). Remarkably, it has been associated with obesity and insulin-resistance, as evidenced in PubMed articles 18430367 and 18381574. Additionally, this enzyme has been implicated in inflammatory arthritis, where it exerts its influence by modulating inflammatory cytokines, as suggested by a PubMed article (PubMed:19660107). Intriguingly, its inhibitory action on anchorage-independent cell proliferation has been observed in a study (PubMed:19787193).

Tissue specificity

Ubiquitous. Highly expressed in adipose tissue. Expressed in placenta, lung, heart and prostate. Detected at lower levels in liver, skeletal muscle, pancreas, testis and small intestine. Highly expressed in joints of patients with rheumatoid arthritis and localized with CD68 cells, a marker for macrophages.

Celluar localization

Endosome;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;

UniPort

Q687X5