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Rabbit Anti-Id1 Polyclonal Antibody#abs133269

Please note that the price provided above is for your reference only. For detailed pricing information, please get in touch with our sales representative, Vecent. Western blot analysis Id1 using Jurkat whole cell lysates This product is for research use only, not for use in diagnostic prodecures...

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Description

Catalog-specification	Delivery time	USD price
abs133269-100ug	1-2 Weeks	301.0
abs133269-50ug	1-2 Weeks	201.0

Please note that the price provided above is for your reference only. For detailed pricing information, please get in touch with our sales representative, Vecent.

Overview
catalog	abs133269
Other names	ID1, also known as inhibitor of DNA binding 1, is a crucial DNA-binding protein inhibitor and a member of the bHLHb24 protein class. It is alternatively named dJ857M17.1.2 and is classified as a dominant negative helix-loop-helix protein. ID1 serves as a key regulator in various biological processes. Moreover, it plays a significant role in inhibiting DNA binding and is therefore referred to as a DNA binding protein inhibitor ID1. Another nomenclature used for ID1 is DNA-binding protein inhibitor ID-1. This dominant negative helix-loop-helix protein, ID1, is critical for modulating gene expression and cellular differentiation. It is prominently expressed in different tissues and is commonly denoted as ID1_HUMAN. The Inhibitor of Differentiation 1, or ID 1, is a vital factor in controlling the differentiation process and behaves as a dominant negative helix-loop-helix protein. Overall, ID1 is an important regulator involved in DNA binding inhibition and the determination of cell fate.
Source	Rabbit
Specificity	The Id1 Antibody is capable of identifying total Id1 levels in the organism's system. This implies that the detection of endogenous levels of total Id1 relies primarily on the effectiveness of the Id1 Antibody. Consequently, the Id1 Antibody plays a vital role in the analysis and understanding of endogenous levels of total Id1 in the body.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit
Antigen	Id1
Application	The recommended dilution for Western blotting is between 1:1000 – 3000, while for immunohistochemistry it is 1:200. For immunocytochemistry/immunofluorescence and ELISA (peptide), the suggested dilutions are between 1:20000 – 1:40000. You can achieve consistent and reliable results by following these guidelines.
Immunogen	A synthesized peptide derived from human Id1.
MW	22kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. This process aimed to improve the purity level of the antiserum and make it more effective in carrying out its intended function. Peptide affinity chromatography is a highly efficient method commonly used in the production of high-quality antibodies and other protein-based products. SulfoLink™ Coupling Resin is a type of chromatography resin that functions by coupling a peptide or protein to the surface of the resin, which then behaves as an affinity matrix for the target molecule.
Clonality	Polyclonal Antibody
Stability & Storage	Ensure that the storage temperature is maintained at -20 °C for a duration of one year to preserve the quality of the product. To avoid any damage or degradation, it is advised to refrain from subjecting the product to repeated freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).
Tissue specificity	Expression correlates with proliferation in some types of cells.
Celluar localization	Cytoskeleton;Golgi apparatus;Nucleus;
UniPort	P41134