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Rabbit Anti-FAS Polyclonal Antibody#abs131441

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Description

Catalog-specification	Delivery time	USD price
abs131441-50ug	1-2 Weeks	201.0
abs131441-100ug	1-2 Weeks	301.0

Overview
catalog	abs131441
Description	The receptor for TNFSF6/FASLG is responsible for triggering apoptosis. When activated, the receptor recruits the adapter molecule FADD which then brings caspase-8 into play. The resulting death-inducing signaling complex (DISC) is then able to activate caspase-8 and set off a cascade of caspases - cysteine proteases that perform apoptosis. By breaking down specific proteins at specific sites, these proteases bring about the death of the cell. This process is essential for maintaining cellular health and preventing the growth of cancerous cells.
Other names	The Apoptosis antigen 1, also known as ALPS 1A, APO 1, or CD 95, is a cell surface antigen that plays a crucial role in apoptosis. It belongs to the TNF receptor superfamily, member 6, and is often referred to as the Fas cell surface death receptor or FAS antigen. Delta Fas, APT1, and FASTM are also alternate names for the protein. The Apo 1 antigen is involved in the initiation of programmed cell death, making it an important regulator of physiological processes such as tissue homeostasis and the immune response. Dysregulation of the Apoptosis antigen 1 has been linked to various diseases, including cancer, autoimmune disorders, and immunodeficiencies. The Apoptosis-mediating surface antigen FAS is expressed on many cell types, including immune cells, and binds specifically to its ligand, FASL. This binding induces Fas-mediated apoptosis, which is crucial for the elimination of damaged or infected cells. In conclusion, the Apoptosis antigen 1 plays a critical role in programmed cell death and is essential for maintaining tissue homeostasis and immune function. Its dysregulation has been associated with a variety of diseases and understanding its mechanism of action is important for developing new therapeutic interventions.
Source	Rabbit
Specificity	The detection of endogenous levels of total FAS is possible with the FAS Antibody.
Species Reactivity	Human;Mouse;Rat
Antigen	FAS
Application	IHC 1:50-1:200, WB 1:500-1:2000, ELISA (peptide) 1:20000-1:40000. These are the recommended dilutions for various immunoassays. The dilution of 1:500 to 1:2000 is commonly used for Western blotting (WB), while for immunohistochemistry (IHC), a dilution range of 1:50 to 1:200 is suggested. In ELISA using peptides, the recommended dilution is 1:20000 to 1:40000. It's important to follow these guidelines to achieve accurate and reliable results when performing these assays.
Immunogen	A synthesized peptide derived from human FAS.
MW	52 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. A highly similar version could be: The antiserum was subjected to peptide affinity chromatography purification through the use of SulfoLink™ Coupling Resin.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Tissue specificity	Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Posttranslational modification	N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
Celluar localization	Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus;Plasma Membrane;
UniPort	P25445