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Rabbit Anti-Actinin Alpha 2/3 Polyclonal Antibody#abs131344

Description

Catalog-specification	Delivery time	USD price
abs131344-100ug	1-2 Weeks	301.0
abs131344-50ug	1-2 Weeks	201.0

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Overview
catalog	abs131344
Description	This bundling protein is believed to serve as an anchor for F-actin, linking it to various structures within the cell. It plays a crucial role in maintaining the integrity and organization of the actin cytoskeleton, facilitating its bundling function.
Other names	Alpha-actinins are actin-binding proteins that play a crucial role in the organization and stabilization of the actin cytoskeleton. One of the major isoforms, alpha-actinin 2 (ACTN2), is predominantly expressed in skeletal muscle. It acts as an F-actin cross-linking protein, facilitating the formation of a network of actin filaments. Mutations in the ACTN2 gene have been associated with certain forms of muscular dystrophy, such as CMD1AA. Another isoform, alpha-actinin 3 (ACTN3), is also found in skeletal muscle and contributes to the structure and function of the sarcomere. However, it is worth noting that some individuals have a natural deficiency of ACTN3 due to a common genetic variant. Despite their similarities, ACTN2 and ACTN3 exhibit distinct expression patterns and functional roles in skeletal muscle development and performance.
Source	Rabbit
Specificity	The Actinin α 2/3 Antibody is capable of detecting overall levels of Actinin α 2/3 that are present within an organism. This function specifically targets the endogenous factors that can impact Actinin α 2/3, allowing users to gain insightful knowledge into the behavior of these molecules within their system. By identifying and tracking changes in Actinin α 2/3 levels, researchers can learn more about how these molecules interact and function within the context of their research.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Chicken;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	Actinin alpha 2/3
Application	For Western blotting, the recommended dilution range is 1:500 to 1:2000, while for immunohistochemistry the range is 1:50 to 1:200. When using the protein in immunofluorescence, it is suggested to dilute it to a range of 1:100 to 1:500. For ELISA assays using the peptide, we suggest diluting the protein to a range of 1:20000 to 1:40000. These dilution recommendations should be followed in order to obtain the most accurate results when using this protein in various applications.
Immunogen	Peptide synthesis has led to the development of a novel compound derived from human Actinin α 2/3. This synthesized peptide exhibits remarkable similarity to its original source, as it retains the key characteristics of Actinin α 2/3 while undergoing rearrangement at the molecular level. Consequently, this peptide represents a significant breakthrough in the field of biotechnology, paving the way for potential therapeutic applications and further exploration of its biological properties. The utilization of peptide synthesis techniques has thus enabled the creation of a highly similar variant of Actinin α 2/3, opening up new avenues for scientific research and medical advancements.
MW	103 kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Tissue specificity	Expressed in both skeletal and cardiac muscle.
Posttranslational modification	Ubiquitinated by FBXL22, leading to proteasomal degradation.
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;
UniPort	P35609/Q08043