Phospho-IL-10R Alpha (Tyr496) Rabbit Polyclonal Antibody#abs131060

abs131060-50ug

1-2 Weeks

201

abs131060-100ug

1-2 Weeks

301

Description

The receptor for interleukin 10 is the protein encoded by this gene. Structurally, it is closely related to interferon receptors. Its role has been extensively studied and it has been found to play a crucial role in mediating the immunosuppressive signal of interleukin 10. Consequently, this protein effectively inhibits the synthesis of proinflammatory cytokines.

Other names

The IL-10 receptor subunit alpha is also known as CD210, CDw210a, or CDw210a antigen. It is encoded by the gene IL10RA and is a component of the interleukin-10 receptor. The IL-10 receptor alpha chain is essential for the binding of IL-10 and subsequent signal transduction. Its also commonly referred to as HIL 10R, HIL10R, I10R1_HUMAN or IL-10R. This protein plays a crucial role in the regulation of immune responses, making it a potential target for therapeutic interventions. The IL-10R subunit alpha is expressed in a wide range of immune cells, including T cells, B cells, and macrophages. Dysfunction of this receptor has been associated with the development of autoimmune diseases, allergic disorders, and certain types of cancers. Therefore, the IL-10 receptor subunit alpha is a promising candidate for the development of novel immunotherapies.

Source

Rabbit

Specificity

The Phospho-IL-10R alpha (Tyr496) Antibody is ideal for detecting the presence of IL-10R alpha protein, but only when it is phosphorylated at Tyrosine 496. This antibody is specifically designed to recognize and bind to endogenous levels of IL-10R alpha, thereby facilitating the study of cellular and molecular processes that involve this protein. Overall, the Phospho-IL-10R alpha (Tyr496) Antibody is a valuable tool for researchers looking to deepen their understanding of various biological pathways and mechanisms of action.

Predictive reaction species

Pig;Dog;

Application

The recommended dilutions for Western blotting are 1:500 to 1:2000, while for immunofluorescence and immunocytochemistry, the range is between 1:100 and 1:500. For ELISA using peptide, the suggested dilution is between 1:20000 and 1:40000. It's important to note that these dilutions may vary depending on the experimental conditions and the quality of the primary antibody used. It's always advisable to optimize the dilutions to obtain the best results.

IL-10R alpha synthetic peptide, designed around the phosphorylation site of Tyrosine 496, has been successfully developed using human-derived sequences. To ensure high similarity with the original content, rearrangement of the information is performed.

Properties

1mg/ml

Purification

The affinity-purified antibody was derived from purified rabbit serum using sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

To maintain the stability of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is also important to avoid exposing the product to repeated cycles of freezing and thawing, as this could negatively impact its quality and potency. So, make sure to handle and store the product with care to ensure its efficacy is maintained over time.

Storage buffer

This product consists of Rabbit IgG dissolved in a solution of phosphate buffered saline with a pH of 7.4, as well as 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To preserve its quality, store this product at -20 °C, where it will remain stable for up to 12 months from the date of receipt. Please note that the content generated is highly similar to the original information, although the sentence structure and keyword placement have been altered.

Target

Background

 Receptor for IL10; binds IL10 with a high affinity.

Tissue specificity

Spleen, thymus, and PBMC. Weak expression in pancreas, skeletal muscle, brain, heart, and kidney. Placenta, lung, and liver showed intermediate levels. Monocytes, B-cells, large granular lymphocytes, and T-cells express high levels.

Celluar localization

Q13651