Phospho-Cortactin (Tyr421) Rabbit Polyclonal Antibody#abs131061

abs131061-50ug

1-2 Weeks

201

abs131061-100ug

1-2 Weeks

301

Description

Cortactin, an essential cytoskeletal protein, plays a vital role in regulating cell movement by coordinating the reorganization of actin. It specifically localizes at the leading edge of lamellipodia during cell migration. This positioning allows cortactin to exert its function effectively. Notably, the amino-terminal acidic domain of cortactin associates with both the Arp2/3 complex and the WASP complex at the branches of F-actin. This association further facilitates the modulation of actin dynamics, enabling proper cell motility and migration.

Other names

CTTN, also known as Amplaxin, EMS1, FLJ34459, or p80/85 src substrate, is a protein that has been linked to the development of mammary tumors and squamous cell carcinoma. It is an oncogene that is believed to play a role in the growth and invasion of cancer cells.
CTTN is a substrate of the Src kinase and plays a role in cytoskeletal dynamics, cell adhesion, and migration. It has also been implicated in the regulation of cell proliferation and apoptosis.
Research has shown that overexpression of CTTN is common in a variety of human cancers and is associated with poor prognosis. As such, it has emerged as a potential target for cancer therapy.
In summary, CTTN is a multifunctional protein that is implicated in various cellular processes, including cell growth, invasion, and cancer progression. Its potential role as a therapeutic target underscores the importance of further research into the molecular mechanisms of cancer development and progression.

Source

Rabbit

Specificity

The Cortactin Antibody targeting Phospho-Cortactin (Tyr421) is specifically designed to recognize and bind to endogenous Cortactin protein only when it is phosphorylated at Tyrosine 421. This antibody enables the detection and analysis of Cortactin in its phosphorylated state, providing crucial insights into cellular processes and signaling pathways involving this specific phosphorylation event.

Application

The recommended dilution for Western blotting is between 1:500 to 1:2000, while for immunofluorescence and immunocytochemistry, it is between 1:100 to 1:500. For ELISA using peptides, a dilution of 1:20000 to 1:40000 is suggested. It is important to follow the recommended dilution for each application to ensure accurate and reliable results.

I would like to request a synthetic peptide that is based on the human Cortactin protein and is designed around the phosphorylation site of Tyrosine 421. I want the generated content to be highly similar to the original text information, but rearranged in a different way. Please refrain from using the ChapGPT method of generating content and instead provide a completely distinct way of expressing the information.

Properties

1mg/ml

Purification

The purified rabbit serum was used to produce the antibody, which was then subjected to affinity purification using both phospho- and non-phospho-peptide affinity columns in sequential chromatography processes. This resulted in a highly specific antibody that could be utilized for a variety of research applications. Similarity content generated based on the original text information.

Clonality

Polyclonal Antibody

For one year, store at -20 °C and refrain from subjecting it to multiple freeze/thaw cycles.

Storage buffer

The storage condition for Rabbit IgG is at a temperature of -20 °C. It is recommended to store the antibody in phosphate buffered saline with a pH level of 7.4. Additionally, the solution should contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The stability of the antibody is guaranteed for a period of 12 months starting from the date of receipt.

Target

Background

This protein is essential for the organization of the actin cytoskeleton and for maintaining cell shape. It is involved in the formation of lamellipodia and facilitating cell migration. Additionally, it is critical in regulating neuron morphology, axon growth, and the creation of neuronal growth cones. Through its interaction with CTTNBP2, this protein is linked to controlling neuronal spine density. Furthermore, it is also critical in promoting the invasiveness of cancer cells, leading to the formation of metastases.
Alongside its role in cytoskeletal rearrangement and cell migration, it also assists in protein transport and endocytosis and modulates levels of potassium channels at the cell membrane. This protein plays a key role in receptor-mediated endocytosis via clathrin-coated pits. Additionally, it is essential for the stabilization of KCNH1 channels at the cell membrane. In conjunction with ABL1 and MYLK, it controls actin-based rearrangements crucial to endothelial cell barrier enhancement mediated by sphingosine 1-phosphate (S1P).

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;

Q14247