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Rabbit Anti-SENP8 Polyclonal Antibody #abs130215

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Description

Catalog-specification	Delivery time	USD price
abs130215-50ug	1-2 Weeks	201
abs130215-100ug	1-2 Weeks	301

Overview
Description	SENP8 is a crucial protease in the NEDD8 pathway that performs two indispensable functions. First, it converts the full-length NEDD8 protein into its mature form, and second, it removes NEDD8 from specific target proteins like cullins or p53. This enzyme belongs to the peptidase C48 family and plays a vital role in the regulation of various cellular processes. With its unique properties and functions, SENP8 is a critical protein in maintaining the proper functioning of the NEDD8 pathway.
Other names	NEDD8-specific protease 1, also known as cysteine 2 or deneddylase 1, is a vital protein involved in the regulation of cellular processes. This protease, encoded by the SENP8 gene, plays a crucial role in the removal of NEDD8 from target proteins. Its alternative names include deneddylase-1, FKSG8, HsT17512, NEDD8 specific protease cysteine 2, NEDP1, PRSC2, SENP8_HUMAN, Sentrin / SUMO specific protease SENP 8, Sentrin-specific protease 8, and many others. The function of SENP8 is to hydrolyze the bond between NEDD8 and its target proteins, thereby regulating NEDD8 conjugation and deconjugation processes. By modifying target proteins with NEDD8, this protease influences various cellular pathways, including protein degradation, cell cycle progression, and DNA repair. It is an essential component of the SUMO/sentrin peptidase family, specifically responsible for NEDD8-specific modifications. Understanding the role of SENP8 is crucial for gaining insights into cellular mechanisms and potentially developing therapeutic interventions. Further research on this protease and its interaction with NEDD8 and other proteins is needed to unravel its complete functions and implications in human health and diseases.
Source	Rabbit
Specificity	The total SENP8 levels in the body can be accurately detected using the SENP8 antibody, which specifically targets the endogenous SENP8 protein. The antibody allows researchers to measure the concentration of SENP8 without interfering with its natural levels. By utilizing this antibody, scientists are able to study the role and function of SENP8 in various biological processes.
Species Reactivity	Human;Mouse;Rat
Application	For Western blotting, it is recommended to dilute the antibody at a range of 1:500 to 1:3000. When used for immunohistochemistry, the antibody should be diluted at 1:50 to 1:200. For immunofluorescence and immunocytochemistry applications, the antibody should be diluted at 1:100 to 1:500. For ELISA using peptides, a dilution range of 1:20000 to 1:40000 is suggested. It is important to follow the recommended dilutions to achieve optimal results in your experiments.
Immunogen	A synthesized peptide derived from human SENP8.
Properties
Concentration	1mg/ml
Purification	The process of purifying the antiserum involved utilizing SulfoLink™ Coupling Resin for peptide affinity chromatography. This method was chosen to selectively isolate the desired components of the antiserum, resulting in a highly purified final product.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure preservation, keep the store at -20 °C for a duration of one year. It is important to avoid subjecting it to freeze/thaw cycles multiple times.
Storage buffer	This product contains Rabbit IgG suspended in a solution of phosphate buffered saline at pH 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and is stable for a period of 12 months from the date of receipt.
Target
Background	This particular protease plays a critical role in the NEDD8 pathway by performing two essential functions. Firstly, it is responsible for processing full-length NEDD8 and converting it into its mature form. Secondly, it assists in the deconjugation of NEDD8 from targeted proteins, such as cullins or p53. Its role in these crucial processes highlights the significance of this protease in maintaining the proper functioning of the NEDD8 pathway.
Tissue specificity	Broadly expressed, with highest levels in kidney and pancreas.
Celluar localization	Cytosol;Nucleus;
UniPort	Q96LD8