Phospho-SMC1 (Ser957) Rabbit Polyclonal Antibody#abs131064

abs131064-50ug

1-2 Weeks

201

abs131064-100ug

1-2 Weeks

301

Description

The cohesin multiprotein complex plays a crucial role in maintaining the cohesion of sister chromatids, and SMC1 is one of its essential components. Although most cohesin complexes disassociate from chromosomes during mitosis, those present at the kinetochore are retained. SMC1 is believed to be a critical element in the functional kinetochores. In summary, SMC1 is a crucial protein component of the cohesin complex, indispensable for maintaining proper chromosome segregation during cell division. Its role in kinetochore function is critical for ensuring genetic stability in cells.

Other names

SmcB, also known as SMC-1-alpha or Structural maintenance of chromosomes 1A, is a protein that plays a crucial role in the segregation of mitotic chromosomes. It is encoded by the KIAA0178 gene and is also referred to as Sb1.8 or DXS423E. SmcB belongs to the family of SMC proteins, which are involved in the structural maintenance of chromosomes in both prokaryotic and eukaryotic organisms.
Structural maintenance of chromosomes 1A is responsible for ensuring that each daughter cell receives the correct number of chromosomes during cell division. SmcB accomplishes this by binding to DNA and forming large protein complexes that help organize and separate the chromosomes. These complexes also play a role in repairing damaged DNA and regulating gene expression.
SmcB has been shown to be essential for normal development and growth, and mutations in the SMC1A gene have been linked to a number of genetic disorders such as Cornelia de Lange syndrome and a form of intellectual disability known as the SMC1A syndrome. Understanding the function of SmcB and other SMC proteins is critical for developing new treatments for these and other genetic diseases.

Source

Rabbit

Specificity

The Phospho-SMC1 (Ser957) Antibody is specifically designed to detect the presence of SMC1 when it is phosphorylated at Serine 957. It has the ability to differentiate between endogenous levels of SMC1 that are phosphorylated at this specific site and those that are not phosphorylated. This antibody is a valuable tool for researchers who are interested in studying the mechanisms that regulate the phosphorylation of SMC1, as well as the downstream effects of this modification. Its specificity and sensitivity make it an ideal reagent for experiments that require precise detection of phosphorylated SMC1.

Predictive reaction species

Pig;Sheep;Dog;Bovine;

Application

The recommended dilutions for this antibody are WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, and ELISA (peptide) 1:20000-1:40000. To ensure optimal results, it is important to use the appropriate dilution for each application. With WB, dilute the antibody in a solution of 1X Tris-buffered saline with 0.1% Tween-20 and 5% BSA. For IHC, use a diluent containing 1% BSA in PBS. For IF/ICC, dilute the antibody in a solution of 1X PBS with 1% BSA and 0.1% Triton X-100. Finally, for ELISA (peptide), dilute the antibody in a coating buffer containing 15mM sodium carbonate and 35mM sodium bicarbonate. By following these guidelines, the antibody will perform optimally and provide accurate and reliable results for your experiments.

A synthetic peptide has been developed from the human protein SMC1, which centers around the phosphorylation site of Serine 957. To create a highly comparable description, the original text information must be rearranged and presented in a different form.

Properties

1mg/ml

Purification

The antibody was extracted by purifying rabbit serum using a sequential chromatography method, which involved passing the serum through both phospho- and non-phospho-peptide affinity columns to ensure optimal purification.

Clonality

Polyclonal Antibody

To preserve the quality of the product, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to take precautions and avoid subjecting the product to repeated cycles of freezing and thawing. This will ensure that the product remains in optimal condition and can be used effectively for its intended purpose. Remember to always follow the storage instructions provided to maximize the shelf life of the product.

Storage buffer

The recommended storage conditions for Rabbit IgG are as follows: store at -20 °C in a solution containing phosphate buffered saline, pH 7.4, with 150 mM NaCl. Additionally, the solution should have 0.02% sodium azide and 50% glycerol. It is crucial to note that the stability of this IgG is guaranteed for a period of 12 months from the date of receipt.

Target

Background

 Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Celluar localization

Cytoskeleton;Cytosol;Nucleus;

Q14683