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Rabbit Anti-FASN Polyclonal Antibody#abs135626

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Description

Catalog-specification	Delivery time	USD price
abs135626-100ug	1-2 Weeks	301.0
abs135626-50ug	1-2 Weeks	201.0

Overview
catalog	abs135626
Description	Fatty acid synthase (FASN) is an enzyme that plays a crucial role in the synthesis of long-chain fatty acids. It operates as a homodimer with seven distinct catalytic functions and is primarily active in the liver, where it produces lipids for export to metabolically active tissues or storage in adipose tissue. However, in most other human tissues, FASN expression is minimal as these tissues rely on circulating fatty acids for lipid synthesis. Interestingly, an increased expression of FASN has been observed in various human carcinomas. For instance, in breast cancer, the levels of FASN have been found to be directly correlated with the size of breast tumors. Similar patterns have been observed in lung and prostate cancers as well, where FASN expression has been identified as a predictor of poor prognosis. In fact, studies have shown that inhibiting FASN can specifically target and kill human cancer cells, highlighting its potential as a therapeutic target for cancer treatment. Given the associations between FASN and cancer, as well as metabolic syndrome, there is growing interest in exploring FASN as a diagnostic marker and a potential target for therapeutic interventions. By understanding and manipulating the activity of FASN, we may be able to develop new strategies for diagnosing and treating cancer, as well as managing metabolic disorders.
Other names	Here are a few options for generating a highly similar content by rearranging the original text information: 1. Fatty acid synthase (FAS) is an important enzyme involved in fatty acid synthesis. It is also known as [Acyl-carrier-protein] S malonyltransferase. Other names for this enzyme include FASN, MGC14367, and MGC15706. FAS plays a crucial role in the biosynthesis of fatty acids by catalyzing various reactions such as acetylation, malonylation, dehydration, reduction, and synthesis. 2. One of the key enzymes in fatty acid biosynthesis is [Acyl-carrier-protein] S acetyltransferase. It works in conjunction with other enzymes like 3-oxoacyl-[acyl-carrier-protein] reductase and 3-oxoacyl-[acyl-carrier-protein] synthase to produce fatty acids. Additionally, the enzyme Enoyl-[acyl-carrier-protein] reductase plays a significant role in this process. These enzymes are collectively known as Fatty Acid Synthase (FAS) and are crucial for the synthesis of fatty acids in the human body. 3. In the pathway of fatty acid synthesis, the enzyme 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase plays a vital role. It catalyzes the dehydration of 3-hydroxypalmitoyl-[acyl-carrier-protein], an intermediate in the fatty acid synthesis pathway. Another enzyme involved in this pathway is Oleoyl-[acyl-carrier-protein] hydrolase, which hydrolyzes oleoyl-acyl carrier protein. All these enzymes are part of the larger Short chain dehydrogenase/reductase family 27X member 1 (SDR27X1). Please note that the generated content has been rearranged to maintain similarity with the original text information.
Source	Rabbit
Specificity	The FASN Antibody is able to detect total FASN at levels that occur naturally within an organism. Any variations observed can be traced back to changes in endogenous levels.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Bovine;Dog;Horse
Antigen	FASN
Application	The dilution ratio for Western Blot (WB) ranges from 1:500 to 1:2000. For Immunohistochemistry (IHC), the dilution ratio is typically between 1:50 and 1:200. In the case of ELISA using peptides, the recommended dilution ratio is from 1:20000 to 1:40000. These dilution ratios provide an optimal range for achieving accurate and reliable results in WB, IHC, and ELISA assays.
Immunogen	A synthesized peptide derived from human FASN.
MW	272kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
Tissue specificity	Ubiquitous. Prominent expression in brain, lung, and liver.
Celluar localization	Cytosol;Extracellular region or secreted;Golgi apparatus;Mitochondrion;Plasma Membrane;
UniPort	P49327