Rabbit Anti-Lamin B Polyclonal Antibody#abs131244

abs131244-50ug

1-2 Weeks

201

abs131244-100ug

1-2 Weeks

301

Description

Lamins, residing within the nuclear lamina, form a fibrous layer on the inner side of the nuclear membrane and play a crucial role in providing structural support to the nuclear envelope. Additionally, they are believed to engage in interactions with chromatin, contributing to the overall organization and functioning of the nucleus.

Other names

Lamin B1, also known as ADLD, is a protein that plays a crucial role in maintaining the integrity of the nuclear envelope. It is encoded by the LMNB1 gene and belongs to the lamin family. Lamin B1 is essential for nuclear structure and function, as it helps support the nuclear membrane and regulates various cellular processes. It is involved in DNA replication, gene expression, and cell division. Mutations in the LMNB1 gene have been linked to autosomal dominant leukodystrophy (ADLD), a progressive neurological disorder characterized by white matter loss in the brain. Understanding the functions and mechanisms of lamin B1 is important for unraveling the pathogenesis of ADLD and other diseases associated with nuclear envelope dysfunction.

Source

Rabbit

Specificity

The Lamin B Antibody is capable of detecting total Lamin B at its natural levels within the body. This antibody is designed to recognize Lamin B within an endogenous context.

Predictive reaction species

Pig;Bovine;Sheep;Rabbit;

Application

The recommended dilutions for Western blotting are 1:500-1:2000, for immunohistochemistry are 1:50-1:200, for immunofluorescence are 1:200, and for ELISA using peptide as the antigen are 1:20000-1:40000. It is important to follow these dilutions to ensure accurate and reliable results in your experiments.

A synthesized peptide derived from human Lamin B.

Properties

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography to achieve purification. It was an effective technique for eliminating impurities and obtaining a highly purified sample.

Clonality

Polyclonal Antibody

To ensure the longevity of your product, it is recommended to store it at a temperature of -20 °C for up to one year. It is advised to avoid exposing the product to repeated freeze/thaw cycles, as this may negatively impact its quality. Take the necessary precautions to maintain the integrity of your product and protect its efficacy.

Storage buffer

The storage condition for Rabbit IgG is as follows: it should be kept in phosphate buffered saline at a pH of 7.4, with a concentration of 150mM NaCl. Additionally, it should contain 0.02% sodium azide and stored with 50% glycerol at a temperature of -20 °C. This product is stable for a period of 12 months starting from the date of receipt.

Target

Background

The nuclear lamina, a fibrous layer located on the inner nuclear membrane, contains important components known as lamins. These lamins are responsible for providing a structural framework for the nuclear envelope and are thought to interact with chromatin. Overall, they play a crucial role in the organization and functioning of the nucleus.

Celluar localization

Cytoskeleton;Nucleus;

P20700