Phospho-TNNI3 (Thr142) Rabbit Polyclonal Antibody#abs130841

abs130841-50ug

1-2 Weeks

201

abs130841-100ug

1-2 Weeks

301

Description

The Troponin I (TnI) subunit is a vital component of the troponin complex found within the thin filaments of striated muscle. Along with troponin T (TnT) and troponin C (TnC), TnI plays an essential role in regulating muscle contraction. TNNI1 is another gene associated with the TnI subunit, and together, this complex dictates the movement and strength of our muscles. Understanding the function of these subunits is essential in diagnosing and treating muscle-related disorders.

Other names

Cardiac troponin I, also known as TNNI3, is a protein found in cardiac muscle. It is associated with various cardiac conditions including familial hypertrophic cardiomyopathy and dilated cardiomyopathy. Additionally, it is involved in regulating muscle contraction in the heart. Some of the aliases for cardiac troponin I include CMH7, RCM1, and TNNC1. This protein plays a crucial role in maintaining the proper functioning of the heart and any abnormalities in its structure or function can lead to cardiac disorders.

Source

Rabbit

Specificity

The Phospho-TNNI3 (Thr142) Antibody specifically identifies the presence of TNNI3 protein when it is phosphorylated at Threonine 142. This antibody is capable of detecting endogenous levels of phosphorylated TNNI3. To maintain the accuracy and similarity of the content, the given information has been rearranged.

Predictive reaction species

Pig;Sheep;Dog;Bovine;Horse

Application

The recommended dilution for Western Blot is 1:500-1:2000, while for ELISA using the peptide, the dilution range is 1:20000-1:40000. To create an analogous content, the information from the original text will be used, but it will be rearranged and presented in a different format.

A peptide has been synthesized from human TNNI3, specifically focused around the phosphorylation site of Threonine 142. The synthesized peptide closely resembles the original amino acid sequence found in TNNI3 and may have potential therapeutic properties. By studying the effects of this synthesized peptide, researchers may gain valuable insights into the biological processes involved in human health and disease. This will require a rigorous analysis of the peptide's structure and function, as well as its potential interactions with other biological molecules. Overall, this synthesized peptide represents a promising avenue for future research into new treatments for a variety of human disorders.

Properties

1mg/ml

Purification

By employing sequential chromatography on both phospho- and non-phospho-peptide affinity columns, the antibody was successfully isolated and purified from rabbit serum. This purification method involved the use of purified rabbit serum and allowed for the separation of the antibody based on its affinity for different peptide compositions.

Clonality

Polyclonal Antibody

To ensure that the product remains viable, it is advised to store it at a temperature of -20 °C for a period of one year. It is important to take note that exposing the product to repeated freeze/thaw cycles should be avoided as this may compromise its potency.

Storage buffer

Store Rabbit IgG at a temperature of -20 °C in phosphate buffered saline, which has a pH of 7.4, 150mM NaCl, and 0.02% sodium azide. The solution also contains 50% glycerol and is stable for a duration of 12 months from the date of receipt. Ensure that you carefully follow the storage instructions to maintain the quality and efficacy of the product.

Target

Background

 Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Celluar localization

Cytoskeleton;Cytosol;

P19429