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Phospho-AP-2mu (Thr156) Rabbit Polyclonal Antibody#abs140289

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Description

Catalog-specification	Delivery time	USD price
abs140289-100ug	1-2 Weeks	301.0
abs140289-50ug	1-2 Weeks	201.0

Overview
catalog	abs140289
Other names	Adaptin mu 1, also known as Adaptor-related protein complex 2 mu subunit or AP-2 mu chain, is a protein involved in clathrin-associated processes. It is a subunit of the adaptor protein complex AP-2, which helps to recruit cargo proteins for transport from the plasma membrane via clathrin-coated vesicles. Other names for this protein include Clathrin assembly protein complex 2 medium chain, Clathrin coat adaptor protein AP50, and Clathrin coat-associated protein AP50. In humans, the AP2M1 gene encodes for the AP-2 mu subunit. The protein is composed of 435 amino acids and has a molecular weight of 50 kDa. It forms a complex with other subunits, including beta, sigma, and omega, to make up the complete AP-2 complex. Together, these subunits enable the complex to bind to clathrin-coated vesicles and facilitate their formation and transport. Mutations or dysregulation of the AP2M1 gene have been associated with various diseases, including cancer and neurodevelopmental disorders. Understanding the role of adaptin mu 1 in these processes may offer insights into potential therapeutic targets for these conditions.
Source	Rabbit
Specificity	When examining endogenous levels of AP-2mu, the AP-2mu (Phospho-Thr156) Antibody specifically identifies the protein when it is phosphorylated at the Thr156 site. It is crucial to note that this antibody does not recognize AP-2mu in its non-phosphorylated state at this specific residue. By targeting the phosphorylated form of AP-2mu at Thr156, the antibody allows for precise detection and analysis in biological samples.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;
Antigen	AP-2mu
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	，。： AP-2mu (-Thr156)。，。
MW	49kDa
Properties
Concentration	1mg/ml
purification	The purification process involves isolating the antibody from rabbit serum through sequential chromatography on phospho- and non-phospho-peptide affinity columns. This ensures the purification and enrichment of the antibody.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity).
Celluar localization	Cytosol;Endosome;Extracellular region or secreted;Lysosome;Plasma Membrane;
UniPort	Q96CW1