Rabbit Anti-Smad9 Polyclonal Antibody#abs131190

abs131190-50ug

1-2 Weeks

201

abs131190-100ug

1-2 Weeks

301

Description

The MAD-related family of molecules includes SMAD9, which plays a crucial role in the signaling pathways of serine/threonine kinase receptors belonging to the transforming growth factor beta superfamily. These intracellular proteins have recently been identified and are considered vital components in these signaling pathways.

Other names

Mothers against decapentaplegic homolog 9, also known as MAD homolog 9 or Madh6, is a protein that belongs to the SMAD family. It is encoded by the Smad9 gene and is involved in various signaling pathways, including the TGF-beta signaling pathway. SMAD9 plays an important role in regulating cell proliferation, differentiation, and apoptosis, among other cellular processes. It is expressed in various tissues and is essential for proper embryonic development. SMAD9 has two isoforms, SMAD8A and SMAD8B. As a key player in the TGF-beta signaling pathway, SMAD9 plays a crucial role in maintaining tissue homeostasis and is implicated in various diseases, including cancer and fibrosis. Understanding the function of SMAD9 may lead to the development of novel therapies for these diseases.

Source

Rabbit

Specificity

The Smad9 Antibody is capable of detecting total Smad9 levels present in the body naturally. The antibody specifically targets endogenous Smad9, meaning it can distinguish the protein from other molecules.

Predictive reaction species

Sheep

Application

The dilution range for western blot (WB) is from 1:500 to 1:2000, for immunohistochemistry (IHC) is from 1:50 to 1:200, and for ELISA with peptide is from 1:20000 to 1:40000. These dilutions are used to ensure optimal detection of the target protein in the respective assays. It is important to appropriately dilute the samples and antibodies to achieve reliable and accurate results.

A synthesized peptide derived from human Smad9.

Properties

1mg/ml

Purification

Purification of the antiserum involved the use of SulfoLink™ Coupling Resin packed into a peptide affinity chromatography column. The process resulted in a highly purified antiserum, ideal for its intended application.

Clonality

Polyclonal Antibody

To ensure the quality and longevity of the product, it is recommended to store it for one year at a temperature of -20 °C. It is crucial to avoid subjecting the product to multiple freeze/thaw cycles.

Storage buffer

The storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that Rabbit IgG is stable for a period of 12 months from the date of receipt.

Target

Background

SMAD9 is a type of transcriptional modulator that is activated by bone morphogenetic proteins, also known as BMP. This particular protein is classified as a receptor-regulated SMAD, or R-SMAD for short. Its activation occurs through the action of BMP type 1 receptor kinase, which plays a critical role in the regulation of various cellular processes. As such, SMAD9 is an important mediator of BMP signaling and can influence the expression of many genes that are involved in different aspects of cell biology.

Tissue specificity

Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.

Celluar localization

Cytosol;Nucleus;

O15198