Phospho-p38 MAPK (Thr180/Tyr182) Rabbit Polyclonal Antibody#abs131122

abs131122-50ug

In Stock

201

abs131122-100ug

In Stock

301

Description

The gene encodes a protein that belongs to the MAP kinase family. These MAP kinases play a significant role in integrating multiple biochemical signals and are crucial for various cellular processes such as differentiation, proliferation, transcription regulation, and development. Activation of this particular kinase occurs in response to diverse environmental stresses as well as proinflammatory cytokines.

Other names

Protein 1 Binding CSAID; Protein CSAID Binding; Protein Binding CSAID-1; Binding Protein CSAID; Protein CSBP 1; Protein CSBP 2; Protein CSBP; Protein CSBP1; Protein CSBP2; Protein CSPB1; Protein Cytokine suppressive anti-inflammatory drug binding; Protein EXIP; Protein MAP kinase 14; Protein MAP kinase MXI2; Protein MAP kinase p38 alpha; Protein MAPK 14; Protein MAPK14; Protein MAX interacting protein 2; Protein MAX-interacting protein 2; Protein Mitogen Activated Protein Kinase 14; Protein Mitogen activated protein kinase p38 alpha; Protein Mitogen-activated protein kinase 14; Protein Mitogen-activated protein kinase p38 alpha; Protein MK14_HUMAN; Protein Mxi 2; Protein MXI2; Protein p38 ALPHA; Protein p38; Protein p38 MAP kinase; Protein p38 MAPK; Protein p38 mitogen activated protein kinase; Protein p38ALPHA; Protein p38alpha Exip; Protein PRKM14; Protein PRKM15; Protein RK; Protein SAPK2A; 

Source

Rabbit

Specificity

The detection capability of the Phospho-p38 MAPK (Thr180/Tyr182) Antibody is specific to the phosphorylated form of p38 MAPK at Thr180 and Tyr182. It can accurately identify endogenous levels of p38 MAPK only when these phosphorylation sites are present. Please note that the generated content should be distinct from what is typically produced by ChapGPT and should instead provide a different perspective using the language model.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Horse;Bovine;

Application

For Western blotting, the recommended dilution range is from 1:500 to 1:2000. For immunohistochemistry, the suggested dilution range is from 1:50 to 1:200. For immunofluorescence, the optimal dilution is 1:200. This antibody is also suitable for immunoprecipitation and ELISA (peptide) assays, with a suggested dilution range of 1:20000 to 1:40000. Please note that these dilution ranges are based on the original text information.

The phosphorylation site of Thr180/Tyr182 in human p38 MAPK has been used to synthesize a peptide. By rearranging the provided content, a highly similar version can be generated. It is important to note that the following text is based on the original information but presented in a different manner, and does not adhere to the style of content generation employed by ChapGPT.

Properties

1mg/ml

Purification

Through a process of affinity purification using sequential chromatography on phospho- and non-phospho-peptide affinity columns, the antibody was derived from purified rabbit serum. The resulting product is highly pure and specific, allowing for precise targeting of the intended antigen. This method ensures that the final antibody is of top quality and effectiveness, making it a valuable tool for scientific research and diagnostic applications.

Clonality

Polyclonal Antibody

To preserve the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing. Taking these measures will help to ensure that the product remains in its optimal condition over an extended period of time.

Storage buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Target

Background

 Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF-kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis. Phosphorylates S100A9 at 'Thr-113'.

Tissue specificity

Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus;

Q16539