Rabbit Anti-SNAI2 Polyclonal Antibody#abs131123

abs131123-50ug

1-2 Weeks

201

abs131123-100ug

1-2 Weeks

301

Description

As a member of the Snail family of C2H2-type zinc finger transcription factors, this gene plays a critical role in regulating gene expression. Specifically, it acts as a transcriptional repressor that binds to E-box motifs, thus inhibiting the activity of certain genes. This gene has been linked to the progression of breast carcinoma, where it is thought to repress the transcription of E-cadherin, a protein that is crucial for maintaining the integrity of epithelial tissues.
In addition to its role in regulating gene expression, this gene has also been found to play a role in epithelial-mesenchymal transitions. This process involves a change in the phenotype of cells, from a tightly-packed, polarized epithelial state to a more motile and invasive mesenchymal state. As a result, this gene has been linked to the development of metastatic disease in certain types of cancer.
Interestingly, this gene has also been found to have antiapoptotic activity. Specifically, it may protect damaged cells from undergoing apoptosis, or programmed cell death. This function appears to be mediated by the repression of certain proapoptotic genes, including the Bcl-2 family protein Puma.
Finally, mutations in this gene have been linked to sporadic cases of neural tube defects. This is a condition where the neural tube, a structure that gives rise to the brain and spinal cord, fails to close properly during embryonic development. This can result in a range of developmental abnormalities, including spina bifida and anencephaly.

Other names

The protein Slug, also known as SNA or Snail homolog, is a neural crest transcription factor that plays a crucial role in development and tissue differentiation. It is encoded by the gene DJ710H13.1 or MGC10182. Slug belongs to the Snail family of transcription factors, which also includes Snail homolog 1 and Snail homolog 2 (SNAI1 and SNAI2, respectively). Slug has also been described as SLUGH or SLUGH1/2.
The Zinc finger protein Slug is characterized by a C-terminal zinc finger domain that mediates specific interactions with DNA sequences. This unique feature allows Slug to regulate the expression of genes involved in cell migration, survival, and epithelial-mesenchymal transition processes. These functions make Slug an essential factor in embryonic development, wound healing, and cancer progression.
In summary, Slug is a critical regulatory protein that controls several cellular processes and plays a vital role in development and disease. Its characterization and further study will help us better understand the complex mechanisms behind these biological phenomena.

Source

Rabbit

Application

The recommended dilution for Western blotting is between 1:500 and 1:2000, while for ELISA (peptide) it is between 1:20000 and 1:40000. By following these dilution guidelines, you can ensure accurate and reliable results in your experiments. It is important to note that these dilution ranges may vary depending on the specific assay and experimental conditions, so it is always best to consult the manufacturer's instructions and perform a pilot experiment to optimize the dilutions for your particular needs. With proper care and attention, you can obtain high-quality data from your Western blotting and ELISA experiments, furthering our understanding of biological processes and disease mechanisms.

A synthesized peptide derived from human SNAI2 .

Properties

1mg/ml

Purification

Peptide affinity chromatography using SulfoLink™ Coupling Resin was employed for the purification of the antiserum. To obtain a similar content but with a different arrangement, it can be stated that the SulfoLink™ Coupling Resin was used in the process of purifying the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions, it is recommended to store the product at a temperature of -20 °C for up to a year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, as this can negatively affect its quality. Therefore, it is best to take all necessary precautions and handle the product with care to ensure its effectiveness and longevity.

Storage buffer

The formulation of rabbit IgG solution contains phosphate buffered saline with a pH of 7.4, along with 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Target

Background

A transcriptional repressor is responsible for modulating both activator-dependent and basal transcription. Its involvement in the generation and migration of neural crest cells is significant. Additionally, it plays a crucial role in mediating the RAF1-induced repression of occludin (OCLN), a TJ protein, leading to the oncogenic transformation of epithelial cells. By binding to the E2-box-containing silencer of BRCA2, this repressor inhibits its expression and recruits CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, it binds to the E-box in the ITGA3 promoter, resulting in the repression of ITGA3 transcription. Furthermore, it regulates the expression of ITGB1 and ITGB4 and controls cell adhesion and proliferation in epidermal keratinocytes. During TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes, it activates the expression of BSG by binding to its E-box2 domain. E-Cadherin/CDH1 transcription is repressed via E-box elements by this repressor. Its involvement in osteoblast maturation is notable, as it binds to the promoters of RUNX2 and SOC9 and may act as a positive and negative transcription regulator, respectively, in osteoblasts. By binding to the E-box regions in the CXCL12 promoter, it exerts its influence on mesenchymal stem cells and osteoblasts. Importantly, this repressor plays a critical role in TWIST1-induced EMT, promoting invasion and metastasis.

Tissue specificity

Expressed in most adult human tissues, including spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Not detected in peripheral blood leukocyte. Expressed in the dermis and in all layers of the epidermis, with high levels of expression in the basal layers (at protein level). Expressed in osteoblasts (at protein level). Expressed in mesenchymal stem cells (at protein level). Expressed in breast tumor cells (at protein level).

Celluar localization

Nucleus;

O43623