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Phospho-AFX1 (Thr455) Rabbit Polyclonal Antibody#abs139946

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Description

Catalog-specification	Delivery time	USD price
abs139946-100ug	1-2 Weeks	301.0
abs139946-50ug	1-2 Weeks	201.0

Overview
catalog	abs139946
Other names	The gene known as AFX, AFX1, or Afxh is also referred to as Fork head domain transcription factor AFX1, Forkhead box O4, or Foxo4. It is encoded by the FOXO4 gene, also known as MLLT7 or Mixed lineage leukemia, translocated to, 7. This gene is associated with the development of certain cancers and is involved in several signaling pathways related to cell growth and survival. Researchers are continuing to study the roles of AFX and FOXO4 in these processes in order to develop new treatments and therapies for these conditions.
Source	Rabbit
Specificity	The AFX1 (Phospho-Thr455) Antibody is designed to detect the presence of endogenous AFX1 protein, but only when it has undergone phosphorylation at Thr455. This antibody is highly specific and will only bind to AFX1 protein molecules that have been modified in this way, allowing for accurate detection and measurement of AFX1 phosphorylation levels. Overall, the AFX1 (Phospho-Thr455) Antibody is a valuable tool for researchers looking to study the biochemical pathways and signaling mechanisms involving this important protein.
Species Reactivity	Human
Antigen	AFX1
Application	The recommended dilutions for using the target protein in various applications are as follows: for Western blotting, it should be diluted at 1:1000-3000; for immunohistochemistry, the appropriate dilution is between 1:50-1:200; for immunofluorescence/immunocytochemistry, a dilution range of 1:100-1:500 is recommended; and finally, for ELISA assays using peptide substrates, the protein should be diluted between 1:20000-1:40000 to obtain optimal results. These dilutions have been tested and verified through extensive experimentation, and should be used as a guideline when conducting experiments involving the target protein.
Immunogen	A peptide that has been synthesized from the human AFX1 with a phosphorylated threonine residue at position 455, is currently available. To generate a similar content, the key factors to focus on are AFX1, the phosphorylated threonine residue, and the synthesized peptide. AFX1 is a human protein that has been found to be involved in multiple cellular processes such as cell proliferation, differentiation, and apoptosis. The phosphorylated threonine residue is an important modification that can affect the function of AFX1. The synthesized peptide is a derivative of AFX1 with the phosphorylated threonine residue at position 455. Such a peptide can be used for various applications such as studying the function of AFX1 or developing therapeutic agents targeting AFX1.
MW	65kDa
Properties
Concentration	1mg/ml
purification	The method used to obtain the antibody involved the purification of rabbit serum through affinity purification, using a series of chromatography techniques on both phospho- and non-phospho-peptide affinity columns. The resulting product is a highly purified antibody with specific targeting capabilities.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is advised to keep it at a freezing temperature of -20°C for up to one year. It is important to avoid repeatedly freezing and thawing the product, as this could compromise its integrity and efficacy. Take care to store the product appropriately to ensure optimal performance.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Tissue specificity	Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
Posttranslational modification	Acetylation by CREBBP/CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. May be phosphorylated at multiple sites by NLK.Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
Celluar localization	Cytosol;Nucleus;
UniPort	P98177