Rabbit Anti-HNF-1beta(TCF-2) Polyclonal Antibody#abs131157

abs131157-50ug

1-2 Weeks

201

abs131157-100ug

1-2 Weeks

301

Description

It is likely that this transcription factor has the ability to bind to the inverted palindrome sequence 5'-GTTAATNATTAAC-3'. In order to produce similar content, the original statement can be rephrased without changing the fundamental information.

Other names

Hepatocyte nuclear factor 1 beta, also known as HNF-1 beta, HNF1B, or TCF2, is a transcription factor that plays an essential role in regulating gene expression during embryonic development and in adult tissues. This homeoprotein is encoded by the HNF1B gene, located on chromosome 17q12. Mutations in this gene are associated with a rare genetic disorder called maturity-onset diabetes of the young type 5 (MODY5), which is characterized by early-onset diabetes, pancreatic hypoplasia, and genitourinary tract anomalies.
HNF-1 beta is widely expressed in different tissues, but its highest levels are found in the liver, kidney, and pancreas. This transcription factor regulates a variety of target genes that are involved in glucose metabolism, lipid homeostasis, cell proliferation, and differentiation. HNF-1 beta binds to DNA sequences known as HNF-1 sites and interacts with other transcription factors, coactivators, and corepressors to promote or repress gene transcription depending on the cellular context.
HNF-1 beta also plays a crucial role in kidney development and function, as it regulates the expression of key genes involved in renal tubulogenesis, ion transport, and water balance. Mutations in the HNF1B gene are associated with a spectrum of renal diseases, including renal cysts and diabetes syndrome (RCAD), renal agenesis, and congenital anomalies of the kidney and urinary tract (CAKUT).
In conclusion, HNF-1 beta is a multifunctional transcription factor that controls diverse biological processes in different organs. Its malfunction can lead to a wide range of genetic disorders affecting carbohydrate and lipid metabolism, kidney and pancreas development, and urinary tract formation. Therefore, a better understanding of the molecular mechanisms underlying HNF-1 beta function and its regulatory networks may provide new opportunities for the diagnosis and therapy of associated diseases.

Source

Rabbit

Specificity

The total HNF-1b (TCF-2) protein at its endogenous level can be detected by the antibody. To create similar content, the wording can be rearranged while conveying the same information as in the original text.

Application

For detecting certain proteins, a western blot can be done at a dilution of 1:500 to 1:1000. An ELISA test using peptides can also be performed at a dilution of 1:20000 to 1:40000. It is important to use appropriate dilution factors to achieve accurate results in these assays.

The peptide sequence derived from Human HNF-1b (TCF-2) that covers amino acids 252 through 256 is R-Q-K-N-P. To create a highly similar content, we can rearrange these five amino acids in different ways such as K-Q-R-N-P or N-P-K-Q-R. It's essential to note that even a small change in the order of amino acids can have a significant impact on the resulting protein structure and function. Therefore, scientists must pay close attention to the exact sequence of amino acids when studying proteins, especially those with critical biological roles like HNF-1b.

Properties

1mg/ml

Purification

Peptide affinity chromatography utilizing SulfoLink™ Coupling Resin was employed to purify the antiserum. For this purpose, the original text explains that the antiserum underwent purification. This purification process involved the use of peptide affinity chromatography with SulfoLink™ Coupling Resin.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid any instances of repeated freeze and thaw cycles to prevent any degradation of the product.

Storage buffer

This product is provided in phosphate buffered saline, pH 7.4, with a concentration of 1.0mg/mL. It does not contain Mg2+ and Ca2+. In addition, the product includes 150mM NaCl, 0.02% sodium azide, and 50% glycerol. These specifications ensure that the product is of high quality and suitable for a wide range of applications.

Target

Background

，5'-GTTAATNATTAAC-3'。，。，ChapGPT。

Celluar localization

P35680