Rabbit Anti-b-Enolase(ENO-3) Polyclonal Antibody#abs131155

abs131155-50ug

1-2 Weeks

201

abs131155-100ug

1-2 Weeks

301

Description

The role of this component appears to be crucial in the development and regeneration of striated muscle.

Other names

Enolase 3, also known as beta-enolase or muscle-specific enolase, is an enzyme that plays a crucial role in glycolysis. It is also referred to as 2-phospho-D-glycerate hydro-lyase or 2-phospho-D-glycerate hydrolyase. ENO3, ENOB_HUMAN, or GSD13 are alternative names for this protein. Its main function is to catalyze the conversion of 2-phospho-D-glycerate into phosphoenolpyruvate in muscle tissues. It is primarily expressed in skeletal muscle cells and is essential for energy production during intense physical activities. This protein is widely studied due to its involvement in various physiological processes related to metabolism and muscle function.

Source

Rabbit

Specificity

The total b-Enolase (ENO-3) protein's endogenous level can be detected using the antibody. To generate highly similar content based on the original text information, the following rearranged content can be provided: The detection of the endogenous level of total b-Enolase (ENO-3) protein is possible through the application of the antibody.

Application

PEPTIDE ELISA has a recommended dilution range of 1:20,000 to 1:40,000, while the Western Blot (WB) assay is typically performed at dilutions of 1:500 to 1:2,000.

One possible rearranged sequence could be "D-G-D-L-R", which also corresponds to a segment within the human b-Enolase protein. This peptide sequence shares a similar pattern of alternating charged and polar residues as the original sequence, which may have functional relevance for enzyme activity or protein-protein interactions. By studying different variations of these short peptide motifs, researchers can gain insights into the structural and biochemical properties of larger macromolecular assemblies.

Properties

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed to purify the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions, it is recommended to store the item at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the item to repeated cycles of freezing and thawing in order to maintain its stability and effectiveness. By following these guidelines, you can ensure that the item remains viable and ready for use when needed.

Storage buffer

1. The solution is prepared as a concentration of 1.0mg/mL in phosphate buffered saline, with the absence of Mg2+ and Ca2+. The pH of the solution is adjusted to 7.4, and it contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol.
2. To create the solution, phosphate buffered saline is used as the base at a concentration of 1.0mg/mL. Mg2+ and Ca2+ ions are deliberately omitted. The pH level is carefully adjusted to 7.4, and it is supplemented with 150mM NaCl, 0.02% sodium azide, and 50% glycerol.
3. Our prepared solution is composed of phosphate buffered saline, excluding Mg2+ and Ca2+, with a concentration of 1.0mg/mL. It has been optimized to a pH value of 7.4 and contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol.
4. The solution provided is a mixture of phosphate buffered saline without Mg2+ and Ca2+, with a concentration of 1.0mg/mL. Its pH level is adjusted to 7.4 and it contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol as additional components.
5. In order to create the aforementioned solution, we combine phosphate buffered saline, excluding Mg2+ and Ca2+, with a concentration of 1.0mg/mL. The pH of the solution is adjusted to 7.4, and it includes 150mM NaCl, 0.02% sodium azide, and 50% glycerol for stabilization purposes.

Target

Background

Striated muscle development and regeneration seem to involve a role for this particular function.

Tissue specificity

The expression of the alpha/alpha homodimer is observed in both embryos and most adult tissues. Conversely, the striated muscle is characterized by the presence of the alpha/beta heterodimer and the beta/beta homodimer. Neurons, on the other hand, display the alpha/gamma heterodimer and the gamma/gamma homodimer. This distribution pattern highlights the diversity of dimeric associations within different cellular contexts.

Celluar localization

P13929