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Rabbit Anti-ROCK1 Polyclonal Antibody#abs131999

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Description

Catalog-specification	Delivery time	USD price
abs131999-50ug	1-2 Weeks	201.0
abs131999-100ug	1-2 Weeks	301.0

Overview
catalog	abs131999
Description	The ROCK1 protein kinase plays a critical role in regulating cell polarity and actin cytoskeleton. It is responsible for the organization of stress fibers and focal adhesions, as well as smooth muscle contraction, neurite retraction, and cell adhesion and motility. The protein kinase achieves these functions through the phosphorylation of various targets, including DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1, and PPP1R12A. It also acts synergistically with FHOD1 to promote SRC-dependent non-apoptotic plasma membrane blebbing. Overall, the ROCK1 protein kinase is a critical player in maintaining cellular structure and function.
Other names	One possible rephrased version could be: Coiled-coil-containing protein kinase 1, also known as p160 Rho-associated coiled coil-forming protein kinase or ROCK1, is a protein implicated in various cellular processes including muscle contraction, cell migration, and cell proliferation. It is encoded by the ROCK1 gene and is predominantly expressed in the brain, heart, and placenta. ROCK1 belongs to the Rho-associated protein kinase family and contains a conserved kinase domain and a unique coiled-coil region that is involved in protein-protein interactions. It functions as a downstream effector of the Rho GTPase signaling pathway and phosphorylates various substrates including myosin light chain and LIM kinase. ROCK1 has been implicated in numerous pathological conditions including cancer, cardiovascular diseases, and neurological disorders. Its overactivation has been shown to promote tumor cell invasion and metastasis, while its inhibition could potentially serve as a therapeutic strategy for cancer treatment. In summary, ROCK1 is a multifunctional protein kinase with diverse physiological and pathological roles. Its molecular structure and signaling pathway regulation make it an attractive target for drug development and disease intervention.
Source	Rabbit
Specificity	The total ROCK1 protein can be detected using the ROCK1 Antibody, which is capable of detecting endogenous levels of ROCK1. In order to generate highly similar content, the rearranged version could be: The ROCK1 Antibody has the ability to detect the total ROCK1 protein at its endogenous levels.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;
Antigen	ROCK1
Application	For Western blotting, the recommended dilutions are 1:500-1:2000. For immunohistochemistry, the suggested dilution is 1:200. If you are conducting immunofluorescence or immunocytochemistry assays, the optimal dilutions are 1:100-1:500. For ELISA using peptide antigens, the ideal dilutions range from 1:20000 to 1:40000.
Immunogen	A synthesized peptide derived from human ROCK1.
MW	160kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.
Tissue specificity	Detected in blood platelets.
Posttranslational modification	Autophosphorylated on serine and threonine residues.Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;
UniPort	Q13464