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Rabbit Anti-GATA5 Polyclonal Antibody#abs131253

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Description

Catalog-specification	Delivery time	USD price
abs131253-100ug	1-2 Weeks	301.0
abs131253-50ug	1-2 Weeks	201.0

Overview
catalog	abs131253
Other names	The GATA binding protein 5, also known as GATA-binding factor 5 or GATA5, is a transcription factor that plays a crucial role in regulating gene expression. This protein, encoded by the GATA5 gene, binds to DNA sequences containing the GATA motif and modulates the expression of downstream target genes involved in various biological processes. GATA5 is primarily expressed in the heart and gut during embryonic development, and it has been implicated in cardiac and gastrointestinal disorders. In particular, mutations in the GATA5 gene have been linked to congenital heart defects and colorectal cancer. Despite its critical role in development and disease, the precise mechanisms by which GATA5 regulates gene expression remain unclear. Future studies will undoubtedly shed more light on the functions of this important transcription factor and its involvement in various biological pathways.
Source	Rabbit
Specificity	The total GATA 5 levels can be detected by the GATA 5 Antibody, which specifically targets the endogenous expression of GATA 5.
Species Reactivity	Human;Mouse
Predictive reaction species	Xenopus;Zebrafish;Chicken;Pig;Dog;Horse;Bovine;Sheep;
Antigen	GATA5
Application	Here are different ways to present the information: 1. For Western blotting, dilute the antibody at 1:500-1:2000. In immunohistochemistry, a dilution range of 1:50-1:200 should be sufficient. For IF/ICC, a dilution of 1:100-1:500 is recommended. The ELISA assay with peptide antigens requires a higher concentration, and the recommended dilution range is 1:20000-1:40000. 2. When using this antibody for Western blotting, the optimal dilution range is between 1:500 and 1:2000. In immunohistochemistry, a dilution range of 1:50 to 1:200 is appropriate. For IF/ICC, it is recommended to dilute the antibody at 1:100 to 1:500. The ELISA assay using peptide antigens requires a higher concentration, and the suggested dilution range is 1:20000 to 1:40000. 3. Depending on the application, the recommended dilution range for this antibody varies. For Western blotting, dilute the antibody at 1:500-1:2000. In immunohistochemistry, a dilution range of 1:50-1:200 should be suitable. For IF/ICC, a dilution of 1:100-1:500 is the recommended range. In ELISA assays that use peptide antigens, a higher concentration is required, and the recommended dilution range is 1:20000-1:40000. 4. Dilution levels for this antibody vary based on the intended application. For Western blotting, a dilution range of 1:500-1:2000 is recommended. In immunohistochemistry, the range of 1:50-1:200 is appropriate. For IF/ICC, dilute the antibody at 1:100-1:500. In ELISA assays that use peptide antigens, the suggested dilution range is 1:20000-1:40000, indicating a higher concentration required for optimal results.
Immunogen	A synthesized peptide derived from human GATA 5.
MW	41 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was achieved through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage, it is recommended to keep the product at a temperature of -20°C for a period of one year. It is important to avoid exposing the product to repeated freeze/thaw cycles as this can negatively impact its quality.
Storage buffer	The rabbit IgG is supplied in a solution containing phosphate buffered saline, with a pH of 7.4, along with 150mM sodium chloride and 0.02% sodium azide. The solution has been supplemented with 50% glycerol, to enhance its stability upon storage. To prevent any deterioration, the product should be stored at -20 °C, and has been shown to remain stable for up to 12 months from the date of receipt.
Target
Background	Transcription factor required during cardiovascular development (PubMed:23289003). Plays an important role in the transcriptional program(s) that underlies smooth muscle cell diversity (By similarity). Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer (PubMed:25543888).
Celluar localization	Nucleus;
UniPort	Q9BWX5