Phospho-AMPK Beta1(Ser181) Rabbit Polyclonal Antibody#abs131119

abs131119-50ug

1-2 Weeks

201

abs131119-100ug

1-2 Weeks

301

Description

AMPKβ1 is a crucial member of the SNF1 kinase family, consisting of an alpha (which catalyzes), beta (which isn't catalytic), and gamma (which also isn't catalytic) subunit. These subunits are 63, 38, and 38kDa, respectively. The primary function of AMPKβ1 is to regulate fatty acid and sterol synthesis by phosphorylating acteyl-coA.

Other names

The gene 1300015D22Rik encodes the 5'-AMP-activated protein kinase subunit beta-1, also known as AMP-activated protein kinase beta subunit or AMPK. This non-catalytic subunit is integral to the AMP-activated protein kinase complex, which plays a critical role in regulating cellular energy metabolism. Other aliases for this protein include AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1, AMP-activated, noncatalytic, beta-1, AMPK beta 1 chain, and AMPK-BETA-1. Additionally, it is referred to by the protein kinase AMP activated non catalytic subunit beta 1 or protein kinase, AMP-activated, beta 1 non-catalytic subunit. It is also known as HAMPKb, PRKAB1, MGC17785, AU021155, and E430008F22.

Source

Rabbit

Specificity

The Phospho-AMPK β1(Ser181) Antibody specifically recognizes AMPK β1 protein when it is phosphorylated at Serine 181. This antibody is designed to detect endogenous levels of phosphorylated AMPK β1, indicating its involvement in cellular processes. By targeting the specific phosphorylation site, this antibody provides valuable insights into the regulatory mechanisms and functions of AMPK β1.

Predictive reaction species

Rabbit;Bovine;Dog;Horse;

Application

The optimal working dilutions for Western blot (WB) range from 1:500 to 1:2000, for immunohistochemistry (IHC) range from 1:50 to 1:200, for immunofluorescence/immunocytochemistry (IF/ICC) range from 1:100 to 1:500, and for ELISA using peptide as the antigen range from 1:20000 to 1:40000. These dilutions should be used as a starting point, and further optimization may be required for individual experiments.

AMPK β1181，，。

Properties

1mg/ml

Purification

Sequential chromatography was used to purify the antibody from purified rabbit serum. This purification process involved affinity purification on phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles during storage.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline, pH 7.4, with 150mM NaCl and 0.02% sodium azide, as well as 50% glycerol. It is recommended that the product be stored at a temperature of -20 °C, and that it be kept for no longer than 12 months from the date of receipt.

Target

Background

 Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

Celluar localization

Cytosol;Nucleus;

Q9Y478