Recombinant Human MIP-1β/CCL4 #abs01125

abs01125-2ug

1-2 Weeks

138

abs01125-10ug

1-2 Weeks

344

abs01125-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

A concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl was filtered through a 0.2 μm filter and then lyophilized. To replicate this process, one would need to obtain a similar solution and then filter it before lyophilizing. The resulting lyophilized product would be a highly concentrated powder that could be easily stored and transported. The pH and salt concentration of the solution would help to maintain the stability and integrity of the product during the lyophilization process. Overall, this is a common method used in the industry for producing high-quality, stable lyophilized products for various applications.

Properties

aa_sequence

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Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

Determined by the LAL method, the concentration of Recombinant Human MIP-1β/CCL4 is below 1 EU/μg.

Activity

The standard and the tested substance exhibited comparable biological activity in a chemotaxis bioassay conducted with human peripheral blood monocytes. The concentration range of 5.0-20 ng/ml was found to support the fully active state of the substance in terms of biological activity.

Reconstitution

Prior to opening this vial, it is advisable to give it a brief centrifugation to ensure that the contents settle at the bottom. For reconstitution, sterile distilled water or buffered solution containing 0.1 % BSA should be used to dilute the contents at a concentration range of 0.1-1.0 mg/mL. To ensure optimal storage for future use, stock solutions should be partitioned into working aliquots and maintained at temperatures ≤ -20 °C. Further dilutions can be made using appropriate buffered solutions.

Stability & Storage

To maintain the stability of this lyophilized preparation, it should be stored at 2-8 °C but for long term storage, -20 °C is highly recommended, with desiccation being preferred. Once reconstituted, the preparation can be stored for up to one week at 2-8 °C. To ensure maximum stability, divide the reconstituted preparation into smaller working aliquots and keep them frozen at -20 °C. It is advisable to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P13236

Gene IDs

6351

References

References

1. Irving SG, Zipfel PF, Balke J, et al. 1990. Nucleic Acids Res. 18:3261-70.

2. Cocchi F, DeVico AL, Garzino-Demo A, et al. 1995. Science. 270:1811-5.

3. Garlisi CG, Xiao H, Tian F, et al. 1999. Eur J Immunol. 29:3210-5.

4. Guan E, Wang J, Roderiquez G, et al. 2002. J Biol Chem. 277:32348-52.