Recombinant Human MIP-1α/CCL3 #abs01123

abs01123-5ug

1-2 Weeks

138

abs01123-20ug

1-2 Weeks

344

abs01123-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl was filtered through a 0.2 μm filter, and then it was lyophilized. Let me know if you need more information or if there's anything else I can assist you with.

Properties

aa_sequence

YLSAVEKYQD EDADRLTLQC CFTIKPGDSS AELTIRQRVS VQEVSFWDPY YFQIEARNSD EAIPKVRSTQ.Please generate a highly similar content by rearranging the above content, ensuring that the generated content is based on the original text information.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The Recombinant Human MIP-1α/CCL3 has been determined to contain a quantity of less than 1 EU/μg using the LAL method. Feel free to ask for any further information related to this topic.

Activity

The biological activity of the standard and the test substance was compared, and it was found that the test substance has full biological activity when compared to the standard. This was determined through a chemotaxis bioassay using human monocytes. The concentration range in which the biological activity was observed for the test substance was between 1.0-10 ng/ml.

Reconstitution

To ensure proper distribution of the contents, we advise briefly centrifuging this vial before opening. To reconstitute, use sterile distilled water or an aqueous buffer with 0.1% BSA to create a concentration of 0.1-1.0 mg/mL. To ensure optimal storage, divide the stock solution into working aliquots and store at ≤ -20 °C. For further dilution, employ the use of appropriate buffered solutions.

Stability & Storage

The lyophilized preparation can be stored at a temperature range of 2-8 °C, but it is advisable to keep it at -20 °C for long-term storage, preferably in a desiccated state. Once reconstituted, the preparation remains stable for up to a week at 2-8 °C. For maximum stability, divide the reconstituted preparation into smaller working aliquots and store them between -20 °C and -70 °C. It is essential to avoid repeated freeze/thaw cycles, as they can affect the stability of the preparation.

Target

Accession

P10147

Gene IDs

6348

References

References

1. Guan E, Wang J, Norcross MA. 2001. J Biol Chem. 276:12404-9.

2. Cocchi F, DeVico AL, Garzino-Demo A, et al. 1995. Science. 270:1811-5.

3. Nakao M, Nomiyama H, Shimada K. 1990. Mol Cell Biol. 10:3646-58.

4. Czaplewski LG, McKeating J, Craven CJ, et al. 1999. J Biol Chem. 274:16077-84.

5. 1991. Hum Gene Ther. 2:87-96.