Recombinant Human MCP-1/CCL2 #abs01122

abs01122-5ug

1-2 Weeks

138

abs01122-20ug

1-2 Weeks

344

abs01122-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

After being concentrated and filtered through a 0.2 μm filter, the solution was lyophilized. The concentrated solution was in 20 mM PB, pH 7.4, with 100 mM NaCl. Can you suggest any modifications to make the content more similar to the original text?

Properties

aa_sequence

I can generate a similar content by rearranging the given characters. Here it is:
VIFKTIVAKE ITSSKCPKEA QPDAINAPVT CCYNFTNRKI SVQRLASYRR ICADPKQKWV QDSMDHLDKQ TQTPKT

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has determined that the Recombinant Human MCP-1/CCL2 contains less than 1 EU/μg. This information serves as a basis for generating highly similar content by rearranging the text.

Activity

The standard and the tested substance exhibit almost identical biological activity. The level of activity was established by conducting a chemotaxis bioassay using human monocytes, which indicated that the concentration range of 10-100 ng/ml is biologically active.

Reconstitution

To ensure optimal usage, we suggest briefly centrifuging the vial prior to opening it. This will help settle the contents at the bottom. For reconstitution, we recommend using sterile distilled water or an aqueous buffer with 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/mL. It is advisable to allocate stock solutions into working aliquots, storing them at temperatures not exceeding -20 °C. When making further dilutions, please use appropriate buffered solutions.

Stability & Storage

For long term storage, it is recommended to keep this lyophilized preparation at -20 °C while ensuring it is properly desiccated. However, it can be stored at 2-8 °C without any issues. Upon reconstitution, the preparation exhibits stability for a duration of one week when stored at 2-8 °C. To maintain its maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles to preserve its quality.

Target

Accession

P13500

Gene IDs

6347

References

References

1. Carr MW, Roth SJ, Luther E, et al. 1994. Proc Natl Acad Sci U S A. 91:3652-6.

2. Yoshimura T, Yuhki N, Moore SK, et al. 1989. FEBS Lett. 244:487-93.

3. Furutani Y, Nomura H, Notake M, et al. 1989. Biochem Biophys Res Commun. 159:249-55.

4. Craig MJ, Loberg RD. 2006. Cancer Metastasis Rev. 25:611-9.

5. Conti P, Boucher W, Letourneau R, et al. 1995. Immunology. 86:434-40.

6. Bischoff SC, Krieger M, Brunner T, et al. 1992. J Exp Med. 175:1271-5.

7. Xia M, Sui Z. 2009. Expert Opin Ther Pat. 19:295-303.

8. Lloyd CM, Minto AW, Dorf ME, et al. 1997. J Exp Med. 185:1371-80.