Recombinant Human Lymphotactin/XCL1 #abs01119

abs01119-5ug

1-2 Weeks

138

abs01119-20ug

1-2 Weeks

344

abs01119-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl was filtered through a 0.2 μm filter before being lyophilized.

Properties

aa_sequence

IQRVGKGGAR NIYQMRAPATL SKRGVVSRTT TGLTKTIAVG LEKTVSVKDV FDCAPWVRTV ITDADRKVRN SKNNTMGTQP GVTLSQTQNA TTVRIRNNVS.Pleask generate a really different content by rearranging the above content, ensuring that the generated content is based on the original text information.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method revealed that the Recombinant Human Lymphotactin/XCL1 contains less than 1 EU/μg. Consider generating the content in a manner that is distinct from the original text, using a language model approach instead of relying on ChapGPT's content generation method.

Activity

The standard and the fully biologically active variant were compared, revealing that the latter exhibited equivalent activity. To determine its biological activity, a chemotaxis bioassay was conducted using human T-lymphocytes. The concentration required to elicit a response was found to be between 10-100 ng/ml.

Reconstitution

Before opening, it is recommended to briefly centrifuge the vial in order to settle the contents at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. It is advisable to divide the stock solutions into smaller working aliquots and store them at temperatures equal to or below -20 °C. For further dilutions, appropriate buffered solutions should be used.

Stability & Storage

For optimal storage of this lyophilized preparation, it is recommended to keep it at -20 °C, preferably in a desiccated condition, for long-term stability. However, it can be stored at 2-8 °C if needed. Upon resuspension, the preparation can be kept for up to one week at 2-8 °C. To maintain its stability, it is recommended to divide the reconstituted preparation into working aliquots and store them at -20 °C to -70 °C. It is advised to avoid repeated freeze/thaw cycles to prevent any degradation of the product.

Target

Accession

P47992

Gene IDs

6375

References

References

1. Kennedy J, Kelner GS, Kleyensteuber S, et al. 1995. J Immunol. 155:203-9.

2. Yoshida T, Imai T, Takagi S, et al. 1996. FEBS Lett. 395:82-8.

3. Yoshida T, Imai T, Kakizaki M, et al. 1998. J Biol Chem. 273:16551-4.