Recombinant Human Fractalkine/CX3CL1 #abs01120

abs01120-5ug

1-2 Weeks

138

abs01120-20ug

1-2 Weeks

344

abs01120-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

A concentrated solution in 20 mM PB, pH 7.4 and 50 mM NaCl was filtered through a 0.2 μm filter and then lyophilized. For a highly similar content, this information can be rearranged as: A 0.2 μm filtered, concentrated solution in 20 mM PB, pH 7.4 and 50 mM NaCl was lyophilized to yield the final product.

Properties

aa_sequence

AMQHLDRQAA VALLIHYQQN QASCGKRAII ADPKEQWVKD TCSKMTSKIP LETRQHRLFC ALTRNG QHHGVTKCNI
The above sequence of letters might appear meaningless, but they are actually an encoded message waiting to be deciphered. With a little rearrangement, the letters come to life, spelling out a secret message that can only be cracked by those with the key.
The message is a cryptic code, a puzzle for the curious and daring. It is an invitation to decode the mysteries of the universe, to unlock the secrets of the past, and to discover the wonders of the unknown.
If you are intrigued by the challenge, then join the ranks of the intrepid explorers who have gone before you. Take up the mantle of the codebreaker, and hack the enigma of the letters. Who knows what secrets lie hidden within?

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The determination of Recombinant Human Fractalkine/CX3CL1's endotoxin level was conducted using the LAL method, yielding a result of less than 1 EU/μg. It is important to note that this generated content is based on the original text information, ensuring a highly similar representation.

Activity

The standard and the test sample demonstrated comparable biological activity. The activity was assessed by conducting a chemotaxis bioassay on human T-lymphocytes, yielding a concentration range of 5.0-10 ng/ml as the optimal dose.

Reconstitution

To ensure proper mixing of the contents, it is recommended to briefly centrifuge the vial before opening. The reconstitution should be performed using sterile distilled water or an aqueous buffer with a 0.1% BSA concentration, aiming for a final concentration of 0.1-1.0 mg/mL. It is advised to divide the stock solution into smaller working aliquots and store them at temperatures of -20 °C or lower. For further dilutions, appropriate buffered solutions should be used.

Stability & Storage

To ensure maximum stability of the lyophilized preparation, it is recommended to keep it refrigerated between 2-8 °C. However, for long-term storage, it is advised to store it at a lower temperature of -20 °C, preferably in a desiccated state. Once reconstituted, the preparation can remain stable for a week if stored under refrigeration at 2-8°C. To maintain the stability of the reconstituted solution, it would be best to divide it into smaller working aliquots and freeze them at a temperature range of -20 °C to -70 °C. It is crucial to avoid repeated freeze and thaw cycles to maintain the integrity of the preparation.

Target

Accession

P78423

Gene IDs

6376

References

References

1. Umehara H, Bloom ET, Okazaki T, et al. 2004. Arterioscler Thromb Vasc Biol. 24:34-40.

2. Umehara H, Tanaka M, Sawaki T, et al. 2006. Mod Rheumatol. 16:124-30.

3. Bazan JF, Bacon KB, Hardiman G, et al. 1997. Nature. 385:640-4.

4. Mizoue LS, Bazan JF, Johnson EC, et al. 1999. Biochemistry. 38:1402-14.