Recombinant Human IP-10/CXCL10 #abs01112

abs01112-5ug

1-2 Weeks

138

abs01112-25ug

1-2 Weeks

344

abs01112-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl was filtered through a 0.2 μm filter before being lyophilized. A highly comparable content can be generated by reordering the original information.

Properties

aa_sequence

SPKMTEKMSR ANKLSLKVIN EKASPNLCRK EKGGKMMITA EVRPCFQSAP IIEKLSELPR VNPQSNISIC VRTVKRSLPV

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that Recombinant HumanIP-10/CXCL10 has less than 1 EU/μg. A highly similar content can be generated by rewording the original information as follows: The level of Recombinant HumanIP-10/CXCL10 has been found to be below 1 EU/μg, as determined by the LAL method.

Activity

The standard and the fully biologically active product showed no significant differences. Its biological activity was assessed using a chemotaxis bioassay on human peripheral blood T-lymphocytes and fell within a 10-50 ng/ml concentration range. The results demonstrated that the product was highly similar to the standard, indicating that it is a reliable and effective treatment option.

Reconstitution

Before opening, we recommend briefly centrifuging this vial to ensure that the contents settle at the bottom. You can reconstitute the contents by adding sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. For storage, divide the solution into smaller working aliquots and keep them at temperatures of -20 °C or below. If further dilutions are necessary, use appropriate buffered solutions.

Stability & Storage

For long-term storage, it is recommended to keep this lyophilized preparation at -20 °C, preferably desiccated. However, it can be stored at 2-8 °C and remains stable. After reconstitution, the preparation retains its stability for up to one week if stored at 2-8 °C. To ensure maximal stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P02778

Gene IDs

3627

References

References

1. Luster AD, Unkeless JC, Ravetch JV. 1985. Nature. 315:672-6.

2. Luster AD, Jhanwar SC, Chaganti RS, et al. 1987. Proc Natl Acad Sci U S A. 84:2868-71.

3. Booth V, Keizer DW, Kamphuis MB, et al. 2002. Biochemistry. 41:10418-25.

4. Dufour JH, Dziejman M, Liu MT, et al. 2002. J Immunol. 168:3195-204.

5. Angiolillo AL, Sgadari C, Taub DD, et al. 1995. J Exp Med. 182:155-62.