Recombinant Human GRO-γ/CXCL3 #abs01105

abs01105-2ug

1-2 Weeks

138

abs01105-10ug

1-2 Weeks

344

abs01105-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl was filtered through a 0.2 μm filter and subsequently lyophilized.

Properties

aa_sequence

NGRQPCTALV SKGKLNIIEI MQNVRSVNSI ETPCAQPHGP VKAKGNKNTL VQMPSAPNLC VTEVIQSLKN LQHLGIQTLC RCQSTVLVAA.
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Carvela shoes, striped shirt, black jeans, silver earrings, and a yellow scarf.
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Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The amount of Recombinant Human GRO-γ/CXCL3 was found to be below 1 EU/μg using the LAL method. Let me create a distinct piece of content by rearranging the provided information, while still reflecting the original text.
The LAL method determined that the Recombinant Human GRO-γ/CXCL3 concentration was less than 1 EU/μg.

Activity

The standard and the fully biologically active form exhibit comparable activity. In order to determine biological activity, a chemotaxis bioassay was conducted using human CXCR2 transfected human 293 cells. The concentration range required for optimal activity was found to be between 10-100 ng/ml. It is important to note that the biological activity of the fully active form closely resembles that of the standard.

Reconstitution

To ensure optimal performance, we suggest centrifuging the vial briefly before opening to settle the contents at the bottom. For reconstitution, use sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. It is recommended to divide the stock solution into working aliquots and store them at -20 °C or below. Dilutions should be performed in buffered solutions that are appropriate for the intended application.

Stability & Storage

For optimal preservation, it is recommended to store this lyophilized preparation at -20 °C, preferably in a desiccated condition. However, it is stable at 2-8 °C as well. Once reconstituted, the prepared solution can be kept for up to one week at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted solution into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles as this may affect its stability.

Target

Accession

P19876

Gene IDs

2921

References

References

1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6.

2. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.

3. Smith DF, Galkina E, Ley K, et al. 2005. Am J Physiol Heart Circ Physiol. 289:H1976-84.

4. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9.