Recombinant Rhesus Macaque IL-8/CXCL8 #abs00958

abs00958-5ug

1-2 Weeks

138

abs00958-25ug

1-2 Weeks

344

abs00958-1mg

1-2 Weeks

6742

Species

Rhesus Macaque

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized to obtain the final product. It is important to ensure that the generated content is highly similar to the original text, while rearranging the wording and structure of the sentence to avoid plagiarism. This process is commonly used in scientific research and is essential for accurately reporting experimental results.

Properties

aa_sequence

ELIKRFCA RECKSASLVK KRENSPVHKI LRQVESNAPI GDPLICKEGR WKRVEEIFVK VHIKAPQNRE PDQNFEKCIR KQVEKVLEKS.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The level of rRhIL-8/CXCL8 is confirmed to be under 1 EU/μg using the LAL method. It is important to note the low concentration of this substance in order to ensure accurate measurements and results.

Activity

The standard and the fully biologically active form exhibit comparable activity levels. To assess its biological activity, human CXCR2 transfected murine BaF3 cells were subjected to a chemotaxis bioassay. The concentration range of the active form spanning from 0.5 to 5.0 ng/ml was determined as indicative of its potency.

Reconstitution

Before opening this vial, it is advisable to briefly centrifuge it in order to settle the contents at the bottom. The reconstitution process requires the use of sterile distilled water or an aqueous buffer containing 0.1% BSA, aiming for a concentration range of 0.1-1.0 mg/mL. To ensure stability, it is recommended to divide the resulting stock solutions into smaller working aliquots and store them at temperatures equal to or below -20 °C. Any further dilutions should be prepared using suitable buffered solutions.

Stability & Storage

To keep this freeze-dried product stable, it is best to store it at 2-8 °C. However, for long term storage, it should be kept at -20 °C and preferably desiccated. If you need to reconstitute the preparation, it will stay stable for up to one week at 2-8 °C. To ensure the greatest stability, divide the reconstituted product into aliquots for work and keep them stored at -20 °C to -70 °C. Don't subject the product to repeated freeze and thaw cycles to avoid compromising its integrity.

Target

Accession

P67813

Gene IDs

613028

References

References

1. Modi WS, Dean M, Seuanez HN, et al. 1990. Hum Genet. 84:185-7.

2. Wolff B, Burns AR, Middleton J, et al. 1998. J Exp Med. 188:1757-62.

3. Utgaard JO, Jahnsen FL, Bakka A, et al. 1998. J Exp Med. 188:1751-6.

4. Minnerly JC, Baganoff MP, Deppeler CL, et al. 1995. Inflammation. 19:313-31.