Recombinant Human GRO-β/CXCL2 #abs01104

abs01104-2ug

1-2 Weeks

138

abs01104-10ug

1-2 Weeks

344

abs01104-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution was filtered through a 0.2 μm filter and then lyophilized. The lyophilization process was carried out in 20 mM PB, pH 7.4, and 50 mM NaCl.

Properties

aa_sequence

PSKGSVNKI KNGKLMKEII VKMPSAPCNL AKQGNKLTI AVEQTCAPHG KSVCQTLQLC RLTELCATPA GNPHGIQSKN
Here is a reorganized version of the original content:
The sequence of letters given above is essentially random and does not form a coherent message.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method indicates that the Recombinant Human GRO-β/CXCL2 contains less than 1 EU/μg. This information pertains to the original text and is expressed in a similar but rearranged manner.

Activity

When compared to the standard, the compound mentioned exhibits full biological activity. This activity was assessed using a chemotaxis bioassay that utilized human CXCR2 transfected human 293 cells. The concentration range for the observed biological activity is 10-100 ng/ml. It is important to note that the compound retains its full biological functionality within this range.

Reconstitution

To ensure optimal usage of this vial, we suggest briefly centrifuging it before opening to bring the contents to the bottom. For reconstitution, sterile distilled water or aqueous buffer containing 0.1% BSA would be appropriate, with a concentration between 0.1-1.0 mg/mL. Stock solutions can be divided into working aliquots and stored at or below -20°C, whereas further dilutions should be carried out in the correct buffered solutions.

Stability & Storage

This freeze-dried product can be stored at 2-8°C, but for long-term storage, it's best to keep it at -20°C while ensuring it's completely dry. Once reconstituted, it can be preserved for up to one week at 2-8°C. However, for maximum stability, it's recommended to divide the reconstituted product into smaller portions and store them at -20°C to -70°C. It's vital to avoid freezing and thawing the product multiple times since this can lead to decreased effectiveness.

Target

Accession

P19875

Gene IDs

2920

References

References

1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6.

2. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.

3. Wolpe SD, Sherry B, Juers D, et al. 1989. Proc Natl Acad Sci U S A. 86:612-6.

4. Iida N, Grotendorst GR. 1990. Mol Cell Biol. 10:5596-9.

5. Pelus LM, Fukuda S. 2006. Exp Hematol. 34:1010-20.