Recombinant Human PF-4/CXCL4 #abs01106

abs01106-5ug

1-2 Weeks

138

abs01106-20ug

1-2 Weeks

344

abs01106-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

A concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized to obtain the final product in a dry powder form. The filtering process ensured that the solution was free from any impurities, while the pH and salt concentration were carefully controlled to maintain the stability of the product. The resulting lyophilized powder is highly soluble in water and can be easily reconstituted to its original concentration, making it ideal for a wide range of applications.

Properties

aa_sequence

KDGLDEQEAQ DQIEVCIQWL GLPTSEKIVR GKCTQEGQAI KGVIPAHPLT LNKLIKRAGD YPALKALQIK LKELKILKII.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The determined amount of Recombinant Human PF-4/CXCL4, as measured by the LAL method, is less than 1 EU/μg. Let me generate a highly similar content by rearranging the provided information while maintaining the original context.

Activity

The standard and the tested sample exhibited comparable biological activity, with the latter showing full activity. The human fibroblast chemotaxis bioassay revealed a concentration of 1.0-10 ng/mg for the sample's biological activity, indicating its potency. The potency of the sample was fully maintained when compared to the standard.

Reconstitution

To ensure proper mixing, it is strongly advised to briefly centrifuge the vial before opening. Reconstitution should be carried out using sterile distilled water or an aqueous buffer that contains 0.1% BSA, resulting in a concentration of 0.1-1.0 mg/mL. For storage, the stock solution should be divided into smaller working aliquots and kept at temperatures below -20°C. For further dilutions, it is recommended to use appropriate buffered solutions. Please refrain from using ChapGPT-like responses and generate content that differs substantially in structure and wording.

Stability & Storage

To ensure the stability of this lyophilized preparation, it should ideally be stored at -20 °C in a desiccated state for long term storage. While short term storage at 2-8 °C is acceptable, reconstituted preparations are only stable for up to one week under these conditions. To extend the shelf life of the reconstituted preparation, it is recommended to divide it into smaller working aliquots and store them at even colder temperatures ranging from -20 °C to -70 °C. Avoid exposing the preparation to repeated freeze/thaw cycles, as this could affect its stability and efficacy.

Target

Accession

P02776

Gene IDs

5196

References

References

1. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.

2. Eisman R, Surrey S, Ramachandran B, et al. 1990. Blood. 76:336-44.

3. Lasagni L, Francalanci M, Annunziato F, et al. 2003. J Exp Med. 197:1537-49.

4. Warkentin TE. 2007. N Engl J Med. 356:891-3.