Recombinant Human NAP-2/CXCL7 #abs01110

abs01110-2ug

1-2 Weeks

138

abs01110-10ug

1-2 Weeks

344

abs01110-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution was filtered through a 0.2 μm filter and then lyophilized. The lyophilized product was obtained from a solution containing 20 mM PB and 50 mM NaCl, with a pH of 7.4. The content generated will be highly similar to the original information, but rearranged in a different format.

Properties

aa_sequence

KSITGPVKHE QVINTLGSEC KGHTVIGLES DPLKCIKARD AKPIKRIKIV QDVITGEALK GACSKKDISG MCIACLRKTN.I have rearranged the original content as follows:

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The concentration of Recombinant Human NAP-2/CXCL7 is determined to be below 1 EU/μg using the LAL method. It is important to note that this information is based on the original text and should be rearranged to generate a highly similar content.

Activity

The standard and the fully biologically active counterpart show significant similarity. To assess their biological activity, a chemotaxis bioassay utilizing human peripheral blood neutrophils was performed. The concentration range of 1.0-10.0 ng/ml was employed to determine the biological activity. This range was found to effectively evaluate the chemotactic properties of the substances.

Reconstitution

It is recommended to briefly centrifuge this vial before opening to ensure that the contents settle to the bottom. Reconstitution should be performed using sterile distilled water or aqueous buffer containing 0.1 % BSA to achieve a concentration of 0.1-1.0 mg/mL. To maintain the integrity of the stock solution, it is advised to aliquot it into working volumes and store at ≤ -20°C. It is also recommended to prepare further dilutions in appropriate buffered solutions.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long term storage, it is best to keep it at -20 °C, preferably in a desiccated condition. Once you reconstitute the preparation, it can remain stable for up to one week at 2-8 °C. To ensure the maximal stability of the product, split the reconstituted preparation into smaller aliquots and store them at temperatures between -20 °C and -70 °C. To prevent degradation, avoid subjecting the product to repeated freeze/thaw cycles.

Target

Accession

P02775

Gene IDs

5473

References

References

1. Hristov M, Zernecke A, Bidzhekov K, et al. 2007. Circ Res. 100:590-7.

2. Majumdar S, Gonder D, Koutsis B, et al. 1991. J Biol Chem. 266:5785-9.

3. Krijgsveld J, Zaat SA, Meeldijk J, et al. 2000. J Biol Chem. 275:20374-81.

4. Piccardoni P, Evangelista V, Piccoli A, et al. 1996. Thromb Haemost. 76:780-5.