Recombinant Human LD78β/CCL3L1 #abs01124

abs01124-5ug

1-2 Weeks

138

abs01124-20ug

1-2 Weeks

344

abs01124-1mg

1-2 Weeks

3891

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized to produce a dried substance for storage and transportation. The resulting lyophilized material is highly similar in composition to the original solution and can be easily reconstituted when needed. This process helps to ensure that the substance remains stable and effective over time, even during extended periods of storage or shipping. Overall, lyophilization is a critical step in the production of high-quality biopharmaceutical and biochemical products.

Properties

aa_sequence

KYVSDLELSA TSSQCSKPSV CCFSYTSRQI APLAADTPTA PQNFIADYFE IFLTKRGRQV CADPSEEWVQ SVWQEESPDAC VGRQRTLKFI EFYDAINFQP ATQRSSTSCY ASDLLESVYK

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The measured amount of Recombinant Human LD78-β/CCL3L1 is below 1 EU/μg, as determined using the LAL method.

Activity

The standard and the fully biological active state were compared, and it was found that the concentration range of 1.0-10 ng/ml displayed similar biological activity. This determination was made using a chemotaxis bioassay with human monocytes. Let's continue the conversation in a completely different manner, not based on generating content from ChapGPT, but rather using a language model to generate distinct speech patterns.

Reconstitution

To ensure optimal results, it is recommended to briefly centrifuge the vial before opening, as this helps bring the contents to the bottom. The product can be reconstituted in sterile distilled water or an aqueous buffer that contains 0.1% BSA, adjusting the concentration to 0.1-1.0 mg/mL. To maintain stability, it is advisable to divide the stock solutions into working aliquots and store them at ≤ -20 °C. When preparing further dilutions, use appropriate buffered solutions.

Stability & Storage

For optimal storage of this lyophilized preparation, it is recommended to keep it at -20 °C in a desiccated state for long-term preservation. However, it can be stored at 2-8 °C and remains stable. Once reconstituted, the preparation retains stability for up to one week when refrigerated at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted solution into smaller portions and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to multiple freeze/thaw cycles.

Target

Accession

P16619

Gene IDs

6349

References

References

1. Irving SG, Zipfel PF, Balke J, et al. 1990. Nucleic Acids Res. 18:3261-70.

2. Hirashima M, Ono T, Nakao M, et al. 1992. DNA Seq. 3:203-12.

3. Miyamoto Y, Machida K, Mizunuma M, et al. 2002. J Biol Chem. 277:28810-4.

4. Struyf S, Menten P, Lenaerts JP, et al. 2001. Eur J Immunol. 31:2170-8.