Recombinant Human SDF-1β/CXCL12β #abs01115

abs01115-2ug

1-2 Weeks

138

abs01115-10ug

1-2 Weeks

344

abs01115-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm membrane and subsequently lyophilized.

Properties

aa_sequence

HMVNWKQYNI EWECIAHPLV LKCNSLTPAQ ALRIKNRFKL NWSVLNRCQK KIPKVKSSFA ECRCLNVLYQ YFHTPCIKLR SPKSYLCQFR.
：
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRF KM.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

As determined by the LAL method, the content reveals that the Recombinant Human SDF-1β/CXCL12β has a concentration of less than 1 EU/μg. To create a highly similar content based on the original information, the following rearrangement can be made: The concentration of Recombinant Human SDF-1β/CXCL12β, determined using the LAL method, is found to be below 1 EU/μg.

Activity

The standard and the fully biologically active substances have been thoroughly compared, and it has been determined that the latter is highly similar to the former. Various tests have been conducted to ensure that it meets all the required parameters, and it has been found to exhibit a strong chemotaxis effect when using PHA and activated human peripheral blood T-lymphocytes. The concentration range of this substance has been determined to be between 20-80 ng/ml. These results prove that this fully biologically active substance is up to the expected standard.

Reconstitution

Before opening the vial, we suggest briefly centrifuging it to ensure the contents settle at the bottom. To reconstitute, mix with sterile distilled water or an aqueous buffer that contains 0.1% BSA. Aim for a concentration of 0.1-1.0 mg/mL. To preserve the solution, divide it into working aliquots and store them at temperatures ≤ -20 °C. Any further dilutions should be made in appropriate buffered solutions. Please note that this response has been generated by language models and may not be based on the original text.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is highly recommended to store it at -20 °C for long term storage, after which it can be desiccated. At a temperature of 2-8 °C, the preparation can still be kept stable, but it is best to keep it at -20 °C for optimal preservation. Once reconstituted, the preparation can maintain its stability for up to one week at a temperature of 2-8 °C. For maximum longevity, it is advised to divide the reconstituted preparation into suitable aliquots for work and storage, and keep them frozen at a temperature range of -20 °C to -70 °C. In order to avoid any harm to the preparation, it is of utmost importance to prevent any repeated freeze/thaw cycles.

Target

Accession

P48061

Gene IDs

6387

References

References

1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500.

2. Yu L, Cecil J, Peng SB, et al. 2006. Gene. 374:174-9.

3. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9.

4. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51.

5. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9.

6. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23.

7. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703.

8. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.

9. Kryczek I, Wei S, Keller E, et al. 2007. Am J Physiol Cell Physiol. 292:C987-95.