Gill's II Hematoxylin Stain

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To prepare the smear, it was first fixed in 95% ethanol for 15 minutes. Then, it was gently rinsed with running water for 30 seconds. Next, the smear was stained with Gill's II hematoxylin solution for a period of 1.5 to 3 minutes. After staining, it was rinsed again with running water. In the next step, the smear was rinsed in either Scott's Tap Water Substitute (BF058) or warm slightly alkaline tap water or diluted ammonia for 15 to 60 seconds. Following this, another rinse with running water was done. To enhance the staining, the smear was dipped in 95% denatured alcohol (BF057) or 95% ethanol for 10 times. Subsequently, it was redyed with Pasteurized solution OG (ST017) for 1.5 minutes. The smear was then again dipped in 95% denatured alcohol or ethanol for 10 times, repeated twice. Continuing the staining process, it was redyed with Pasteurized dye EA (ST017) for a period of 2.5 to 3 minutes and dipped in 95% denatured alcohol or ethanol for 10 times, repeated twice. After that, the smear was rinsed in anhydrous ethanol for 1 minute, repeated twice. Finally, it underwent rinsing in xylene or xylene substitute for 2 minutes, repeated twice, before microscopic examination after cover.
For regression staining, the frozen sections underwent dewaxing and hydration. Then, they were stained with Gill's II hematoxylin for a period of 1.5 to 3 minutes. Following staining, the sections were rinsed with running water. A differentiation solution was applied for 20 to 60 seconds, followed by another rinse with running water. To complete the staining process, the sections were rinsed in either Scott's Tap Water Substitute, warm slightly alkaline tap water, or diluted ammonia for 15 to 60 seconds. After a final rinse with running water, the staining procedure differed based on the purpose. For histology, the sections were restained with Eosin Y (alcohol soluble or water soluble) for 30 to 60 seconds. For cytology, restaining was done with Pasteurized solution OG or EA (ST017) for a period of 1 to 3 minutes. Dehydration, transparency, tablet sealing, and microscopic examination followed for both cases.

Store at room temperature and away from light

Gill Hematoxylin Dyeing Solution (Gill ⅱ)