Rapid Antigen Retrieval Solution For Frozen Sections(5×)

Paraformaldehyde, formaldehyde, and other aldehyde reagents are often used to fix cells or tissues. However, one drawback of this fixation process is the formation of cross-links between proteins. These cross-links can interfere with the binding of antibodies to their target antigens, leading to a weakened staining signal during immunostaining. In some cases, false positive staining results may also be observed. It is important to be aware of this potential issue and consider alternative fixation methods or antigen retrieval techniques to minimize the impact on immunostaining outcomes.
The Frozen Sections Quick Antigen Retrieval Solution (5×) is formulated with various Antigen repair reagents, including SDS, and has a pH value of approximately 7.4. It effectively eliminates protein cross-linking caused by aldehyde fixation reagents and fully exposes epitopes in paraffin sections and other samples. This remarkable improvement greatly enhances the immunostaining effectiveness. Specifically designed for the rapid repair of antigens in frozen sections, this solution requires a mere 5-minute incubation at room temperature.
Antigenic repair is typically required for paraffin sections, whereas frozen sections do not necessitate this step. The improvement of immunostaining performance in paraffin sections can be achieved through antigen repair. Interestingly, numerous studies have also demonstrated significant enhancements in immunostaining for frozen sections. Hence, antigenic repair could be considered when immunostaining of frozen sections proves ineffective. Regardless of whether it is a frozen section or cell slide, the use of paraformaldehyde, formaldehyde, or other aldehyde reagents for sample fixation allows antigen repair to effectively eliminate protein cross-linking, fully expose antigen epitopes, and greatly enhance the immunostaining effect.