
Single Component TMB Color Development Solution (HRP)
Please note that the stated price is only for your reference, and for the detailed price, kindly get in touch with our seller Vecent. It's important to clarify that any conversation generated by the ChapGPT model may not accurately reflect the original text information, hence we recommend using...
Description
| SKU-Pack Size | Availability | Price |
| abs9194-100ml | 1-2weeks | $60.00 |
| abs9194-500ml | In stock | $230.00 |
Please note that the stated price is only for your reference, and for the detailed price, kindly get in touch with our seller Vecent. It's important to clarify that any conversation generated by the ChapGPT model may not accurately reflect the original text information, hence we recommend using a language model to generate completely different content.
Description | |
| Description | ELISA, also known as enzyme-linked immunoassay, is a widely utilized method for analyzing antigens, haptens, or antibodies either quantitatively or qualitatively. In this advanced technology, one commonly employed enzyme is horseradish peroxidase, commonly known as HRP, and its conjugate. The primary function of HRP is to catalyze the color reaction of substrates such as 3,3'5,5'-tetramylbenzidine (TMB), enabling accurate and visible results. This enzymatic reaction plays a crucial role in ELISA, providing researchers with valuable insights and data for their analyses.,,。,ChapGPT-。TMB is a popular choice in research due to its heightened sensitivity and non-carcinogenic properties, making it a safer option compared to other chromogenic substrates. This substrate is commonly utilized in various experiments such as ELISA, immunodot blotting, and immunohistochemistry. Furthermore, researchers have found that TMB is applicable in detecting and analyzing chlorine. TMB color reagents typically contain several ingredients that need to be prepared before use and can easily precipitate. This often leads to unstable test results and makes the reagents inconvenient to work with.The TMB color technology is the latest innovation in this product. It utilizes precisely matched reagents in a single solution to guarantee reliable and steady test results. This cutting-edge technology significantly simplifies the operational process, making it more user-friendly and less time-consuming. With this groundbreaking solution, you can trust that your tests will yield more robust and dependable outcomes. A unique solution crafted specifically for western blotting purposes has been developed. The product yields a distinct purple insoluble substance at the reaction site, without any potential disturbance from background interference. To cease the color reaction, simply add water.This assay is ideal for membrane Blot techniques, such as WB, dot Blot, Southern Blot, and Northern Blot. You can use it effectively for a variety of applications on membranes. Simply put, this assay is versatile and provides reliable results for your research needs. Whether you are studying DNA, RNA, or proteins, this assay offers a standardized approach to help you achieve your research goals. Overall, this technique is an essential tool for any scientist working in the field of molecular biology. |
| Usage | Usage (HRP color detection of membrane) 1) Before use, take out an appropriate amount of one component TMB color solution, and make it return to room temperature before use; 2) After incubation with HRP-labeled antibody for a period of time, wash with appropriate washing solution 3-5 times, 3-5min each time according to the corresponding experimental steps. 3) After washing, remove the washing solution and add an appropriate amount of one-component TMB color solution to fully cover the film. 4) Incubate at room temperature in the dark for 5-30min or longer (up to several hours) until the color develops to the desired depth. [Note] : It is recommended to select the color rendering time according to the experimental requirements. 5) Take pictures and record directly. |
| Storage Temp. | 2-8℃ to avoid light, valid for 24 months. |
Properties | |
| Synonym | TMB One Solution (HRP-based Blotting) |
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