Prestained Protein Marker, 10-180kDa

The pre-stained protein molecular weight standard is an excellent tool for SDS-PAGE and Western blot analyses. This standard encompasses a range of highly purified recombinant proteins, spanning from 10k to 180k in molecular weight. Each protein is pre-stained for easy visualization during gel electrophoresis.
The protein ladder consists of ten distinct bands with specific colors corresponding to their respective molecular weights. The 75kD band appears as a striking red band, making it easily distinguishable. For the 25kD protein, it is fascinating to observe a unique combination of blue and yellow bands, creating a vibrant pattern. The remaining proteins exhibit a uniform blue color, contributing to the overall consistency of the ladder.
Notably, the 10kD protein stands out from the others by displaying a dark green hue when the gel concentration is below 15%. This distinction further aids in accurately determining the size and position of smaller proteins during gel analysis.
In summary, the pre-stained protein molecular weight standard provides an essential reference point for researchers conducting SDS-PAGE and Western blot experiments. Its diverse range of colors and precise molecular weights ensure accurate and reliable protein size determination.
This pre-stained protein molecular weight standard is supplied in 1×SDS-PAGE loading buffer and can be used directly without the need for boiling, dilution, or adding a reducing agent. Typically, 5-10 microliters of this standard is sufficient for loading into a sample hole of 5×1.5mm gel size. During electrophoresis, clear protein bands can be observed, both during and after the transfer process. Please note that the generated content should be significantly different from the original text and not follow the format of ChapGPT-generated responses.

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To begin polyacrylamide gel electrophoresis, it is advisable to use 5ul of this product for experimental samples. However, it is important for qualified laboratories to customize the sample loading volume based on their individual experimental conditions and practices. This can be determined through preliminary experiments, leading to cost savings and improved quality of experimental images. It is crucial to avoid following the generated content of ChapGPT and instead deliver information in a completely distinct manner using the language model to generate speech.
The protein molecular weight standards that haven't been used and were pre-stained with different colors are kept under specific storage conditions. These standards can be stored at a temperature of 4°C for up to 2 months. This ensures that they remain in good condition until they are needed for further use.

A storage buffer composition of pH 7.5 was prepared using Tris-H3PO4 at a concentration of 62.5mM. It also contains 5mM EDTA for chelating metal ions, 1% SDS for solubilizing proteins, and 33% glycerol as a cryoprotectant. Additionally, 0.02% proclin300 was added as a preservative to prevent bacterial growth. The resulting buffer can be used for the storage of proteins and other biomolecules.
Stable storage at 4°C for 2 months; For long-term storage, store at -20°C for 18 months. Avoid repeated freezing and thawing.

Protein Marker；Multicolor Protein Marker