Description | This protease inhibitor product is a potent blend of several commonly used inhibitors such as AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, and Pepstatin. Its broad-spectrum inhibition properties effectively suppress the activity of various proteases found in different tissues and lysates, ranging from primary cells, mammals, animals, plants, bacteria, yeast, and other cells. Notably, this product is free from EDTA, a known inhibitor of metalloproteinases, making it a suitable choice for those studying the active center of metal ion-dependent proteins. Whether you're performing research or conducting experiments, this product is a must-have in your lab for efficient and reliable protease inhibition. |
Usage | 1) Before use, balance at room temperature until it is completely dissolved (Note: DMSO melts slowly at low temperature, it is recommended to melt at 25° C), and vibrate at low speed to ensure the uniformity of each component. 2) Add an appropriate amount of extraction reagent (such as cell or tissue lysate) in the ratio of 1:100 (volume ratio), mix well, and then extract the sample protein.Or according to the purpose of the experiment and the specific cells and tissue culture, the use of the proportion of exploration and optimization.[Note] : According to the recommended ratio of 1:100, 1ml of this product can inhibit the protease activity obtained from 20g wet weight cell lysis. |
