Multicolor Protein Marker, 10-180kDa #abs924

Description

The color pre-stained protein molecular weight standard contains 10 highly purified and pre-stained recombinant proteins from 10k to 180k (10, 17, 25, 33, 43, 55, 72, 95, 130, 180k), of which the 72kD band is orange Red, 10k is a mixture of blue and yellow bands, the remaining bands are blue, and 10k bands are dark green when the glue concentration is below 15%. It is suitable as a protein molecular weight standard for SDS-PAGE and Western blot.
This color pre-stained protein molecular weight standard has been prepared in 1×SDS-PAGE loading buffer and used directly. Do not boil, dilute, or add reducing agent. According to the size of the sample hole, this color pre-stained protein molecular weight standard is usually 5-10 microliters per sample (5×1.5mm gel hole 5ul is enough), which can be observed Clear protein bands during electrophoresis, after electrophoresis, and after transfer.

Usage

1. Dissolve completely after thawing at room temperature and mix gently and thoroughly, do not boil;
2. Take 5ul of this product and experiment samples for polyacrylamide gel electrophoresis at the same time; it is recommended that qualified laboratories use this product for the first time according to their own experimental conditions and experimental habits to determine the appropriate sample loading volume through preliminary experiments. It can save costs and obtain better experimental pictures at the same time;
3. The unused color pre-stained protein molecular weight standards are stored in storage conditions and can be stored for 2 months at 4°C.

Stability & Storage

Storage buffer: 62.5mM Tris-H3PO4, pH7.5, 5mM EDTA, 1%SDS, 33%Glycerol, 0.02% proclin300。
Stable storage at 4°C for 2 months; For long-term storage, store at -20°C for 18 months. Avoid repeated freezing and thawing.

Basic Information