Antibody Diluent For WB
This product is for research use only, not for use in diagnostic prodecures or in human.
Description
| SKU-Pack Size | Availability | Price |
| abs954-100ml | 1-2weeks | $25.00 |
| Description | Primary and secondary antibodies can be effectively diluted and prepared for Western blot using WB special diluent. This diluent is carefully designed with the inclusion of various antibody protectors that enhance the stability and activity of the antibodies. Additionally, it helps to minimize non-specific binding and background coloring, ultimately improving the specificity and sensitivity of the test. Rearranging the given information, we can have the following similar content: |
| Appearance | Solution |
| Protocol | You can directly use this product to dilute your primary or secondary antibody in the appropriate ratios. Each 100 mL of this WB-specific diluent can be diluted 10 times, with a working volume of 10 mL per antibody. The diluted primary or secondary antibody can be stored at 4°C and reused until the desired result is not achieved. Please note that this product has been filtered and sterilized. To maintain its stability and effectiveness, it is important to prevent bacterial contamination during usage. It is recommended to use the product within one month after opening and discard it if any precipitation or bacterial contamination occurs. Moreover, this product does not contain sodium azide, ensuring it will not interfere with the activity of peroxidase-labeled antibodies. It is also free of phosphate, so it will not inhibit the activity of alkaline phosphatase-labeled antibodies. Additionally, the absence of skimmed milk powder in this product ensures that it does not interfere with the activity of biotin-labeled or alkaline phosphatase-labeled antibodies. |
| Operating Instructions | 1) To extend the lifespan of the diluted primary antibody, it is advisable to conduct the incubation at a temperature of 4 °C whenever possible. 2) Kindly note that this product contains preservatives, so it is important to avoid direct contact with the skin. 3) To ensure your safety and well-being, it is recommended to wear a lab coat and disposable gloves while handling this product. |
| Usage | This diluent is specifically designed for diluting primary or secondary antibodies. It should be used directly, without any additional dilution, in the corresponding multiples. For instance, if you have a 10 mL working volume, you can dilute the antibody using 100 mL of this WB-specific diluent, which allows for a 10-fold dilution. The diluted primary or secondary antibody can be stored at 4 °C and reused until you achieve the desired results. However, please discard the diluent if you no longer achieve the desired results or if there is any precipitation or bacterial contamination. It's important to note that this product has already been filtered and sterilized. To enhance its stability and shelf life, it is advisable to avoid any bacterial contamination during handling. Upon opening the product, it is recommended to use it within one month. If you notice any precipitation or bacterial contamination, please discard the product. You can be assured that this diluent does not contain sodium azide, so it will not interfere with the activity of peroxidase-labeled primary or secondary antibodies. Similarly, it does not contain phosphate, which means it will not inhibit the activity of alkaline phosphatase-labeled primary or secondary antibodies. Furthermore, it is free of skimmed milk powder and will not interfere with the activity of biotin-labeled or alkaline phosphatase-labeled antibodies. |
| General Notes | 1. In order to prolong the service life of the diluted primary antibody, the primary antibody should be incubated at 4 ° C as far as possible. 2. This product contains preservatives, please be careful to avoid direct skin contact. 3. For your safety and health, please wear a lab coat and disposable gloves. |
| Overview | |
| Storage Temp. | Store at 4℃ for 12 months. |
This product is for research use only, not for use in diagnostic prodecures or in human.
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