Mouse IL-6 ELISA Kit

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Cat: abs520004

Size: 96T

Mouse IL-6 ELISA Kit

Testing Principle

The Mouse IL-6 ELISA Kit employs a quantitative sandwich enzyme immunoassay method, which includes pre-coating a high affinity plate with a specific anti-mouse IL-6 antibody. Next, biotinylated detection antibodies, standards, and test samples are sequentially added to the wells of the enzyme-labeled plate. Following an incubation period, immune complexes are formed by the combination of IL-6, the solid-phase antibodies, and the detection antibodies. Any unbound material is removed through washing, and then horseradish peroxidase-labeled streptavidin (HRP) is added. After additional washing, a chromogenic substrate is introduced. The color is developed in a dark environment and terminated by adding a stop solution. Finally, the absorbance is determined at 450 nm while using a reference correction wavelength of either 540 nm or 570 nm.

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Mouse IL-6 ELISA Kit composition:

This microplate comes equipped with a capture antibody coating, standard, detection antibody, reagent diluent, Color reagent (A and B), wash buffer, Stop solution, and ELISA plate sealers. All of the components work together to ensure accurate and reliable results in your experiments. Simply follow the provided specifications to achieve optimal performance. This microplate is perfect for a variety of applications and is a valuable tool in any laboratory.

Sensitivity:1.8 pg/mL

Detection range:  15.6 - 1,000 pg/mL

Recovery rate: 70-114%

Storage: 2-8 ° C

The standard curve:

Background: IL-6

The Mouse IL-6 ELISA Kit is a versatile cytokine that is involved in a wide range of biological processes. It is a helical protein that is phosphorylated and can be glycosylated. IL-6 plays an essential role in the body's acute phase response, as well as inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is made up of 183 amino acids and has 39% sequence identity with mouse and rat IL-6. Several isoforms of IL-6 are generated through alternative splicing, some of which may exhibit antagonistic properties. IL-6 is produced by various cells, including fibroblasts, adipocytes, osteoblasts, and synoviocytes. IL-6 levels are usually tightly regulated in the body by glucocorticoids, catecholamines, and secondary sex steroids. Normal human circulating IL-6 levels are around 1 pg/mL, and these levels may increase during the menstrual cycle, certain cancers, or after surgery.

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 Ra, triggering IL-6 Ra association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 Ra are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 Ra elicit responses from gp130-expressing cells that lack cell surface IL-6 Ra. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 Ra is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 Ra but not from other cytokines that use gp130 as a co-receptor.

Mouse IL-6 ELISA Kit, along with TNF-a and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis. IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity. It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis.

Mouse IL-6 ELISA Kit Technical hints: 

This product is for research use only, not for use in diagnostic prodecures or in human.

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