Mouse CCL3/MIP-1 Alpha ELISA Kit

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Cat: abs520019

Size: 96T

Mouse CCL3/MIP-1 alpha ELISA Kit

Testing Principle

The quantitative sandwich enzyme immunoassay technique is employed in this kit. A high affinity plate is coated with a specific anti-mouse MIP-1 alpha antibody. The wells of the enzyme-labeled plate are then loaded with standards, test samples, and biotinylated detection antibodies. Following an incubation period, the MIP-1 alpha present in the samples binds with the solid-phase antibodies and the detection antibodies, resulting in the formation of immune complexes. After removing any unbound material through washing, horseradish peroxidase-labeled streptavidin (HRP) is introduced. Subsequently, a chromogenic substrate is added, and the color develops in a dark environment. To halt the reaction, a stop solution is added, and the absorbance is measured at 450 nm wavelength (with reference correction wavelength of 540 nm or 570 nm).

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

The package includes a 96-well polystyrene microplate that has been coated with the capture antibody, as well as a standard and detection antibody. Additionally, there is a 10x reagent diluent, color reagent A and B, and a 25x wash buffer. To stop the reaction, a stop solution is provided, and the package also includes ELISA plate sealers to prevent any contamination. All of these components are accompanied by a detailed specification, ensuring that everything is of the highest quality and suitable for effective use.

Sensitivity:   1.5 pg/mL

Detection range:   7.81 - 500 pg/mL

Recovery rate:    85.0-111.9%

Storage: 2-8 ° C

The standard curve:

Background: MIP-1 alpha

MIP-1 Alpha (macrophage inflammatory protein 1 Alpha) is a member of the CC or beta chemokine subfamily, originally derived from the conditioned medium of lPS-stimulated mouse macrophage lines. It exhibits chemotactic properties towards various cells such as monocytes, T cells, B cells, and eosinophils.
Through replication/mutation processes, two human MIP-1α genes were generated, resulting in the production of MIP-1α isotypes CCL3/LD78a and CCL3L1/LD78b. These isotypes share a remarkable 94% amino acid sequence homology. While human CCL3/LD78a is a single-copy gene, the copy number of the human CCL3L1/LD78b gene varies among individuals in the population.
Human CCL3L1/LD78b is capable of binding to and eliciting signaling via chemokine receptors CCR1 and CCR5. Notably, CCL3L1/LD78b exhibits higher affinity binding to CCR5, which is also the co-receptor involved in HIV-1 entry into cells. As a result, the copy number variation of CCL3L1 serves as one of several genetic determinants influencing susceptibility to HIV-1.
In summary, MIP-1 Alpha plays a crucial role in cell migration and immune responses, with its human counterparts, CCL3/LD78a and CCL3L1/LD78b, exhibiting slight genetic differences and varying abilities to bind chemokine receptors, particularly CCR5. The genetic variation of CCL3L1 also impacts the susceptibility to HIV-1 infection.

Technical hints: For research use only, not for in vitro diagnosis.

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